Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts

Risa Koda, Takao Numata, Shinji Hama, Sriappareddy Tamalampudi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We demonstrated ethanolysis of rapeseed oil to produce biodiesel fuel using lipase-producing filamentous fungi immobilized on biomass support particles (BSPs) as a whole-cell biocatalyst. We prepared two types of whole-cell biocatalyst: wild-type Rhizopus oryzae producing triacylglycerol lipase (w-ROL) and recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (r-FHL). Both w-ROL and r-FHL successfully catalyzed the ethanolysis of rapeseed oil, and the fatty acid ethyl ester yield was as high as 79% (w-ROL) or 94% (r-FHL). In the case of r-FHL, the residual monoglycerides (MGs) and diglycerides (DGs) were no more than 0.73 and 0.18%, respectively. In addition, r-FHL could be recycled for the ethanolysis of rapeseed oil, retaining over 85% fatty acid ethyl ester yield by the fifth cycle. r-FHL was revealed to be a promising catalyst for biodiesel production using rapeseed oil and ethanol.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalJournal of Molecular Catalysis B: Enzymatic
Volume66
Issue number1-2
DOIs
Publication statusPublished - 2010 Sep 1

Keywords

  • Biodiesel fuel
  • Biomass support particle
  • Ethanolysis
  • Rapeseed oil
  • Whole-cell biocatalyst

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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