TY - JOUR
T1 - Ethanolamine Plasmalogen Suppresses Apoptosis in Human Intestinal Tract Cells in Vitro by Attenuating Induced Inflammatory Stress
AU - Nguma, Ephantus
AU - Yamashita, Shinji
AU - Kumagai, Kei
AU - Otoki, Yurika
AU - Yamamoto, Ayaka
AU - Eitsuka, Takahiro
AU - Nakagawa, Kiyotaka
AU - Miyazawa, Teruo
AU - Kinoshita, Mikio
N1 - Funding Information:
A part of this research was supported by a grant from the Project of NARO Bio-oriented Technology Research Advancement Institution (R&D matching funds on the field for Knowledge Integration and Innovation) and JSPS KAKENHI grant no. JP19K05892.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/2/2
Y1 - 2021/2/2
N2 - Ethanolamine plasmalogen (PlsEtn) is a subtype of ethanolamine glycerophospholipids (EtnGpl). Recently, PlsEtn has attracted increasing research interest due to its beneficial effects in health and disease; however, its functional role in colonic health has not been well established. This study was conducted to determine the mechanism underlying the antiapoptotic effect of PlsEtn in human intestinal tract cells under induced inflammatory stress. Lipopolysaccharide induced apoptosis of differentiated Caco-2 cells, which was suppressed by EtnGpl in a dose-dependent manner. Cells treated with ascidian muscle EtnGpl containing high levels of PlsEtn demonstrated a lower degree of apoptosis, and downregulated TNF-α and apoptosis-related proteins compared to those treated with porcine liver EtnGpl containing low PlsEtn. This indicates that PlsEtn exerted the observed effects, which provided protection against induced inflammatory stress. Overall, our results suggest that PlsEtn with abundant vinyl ether linkages is potentially beneficial in preventing the initiation of inflammatory bowel disease and colon cancer.
AB - Ethanolamine plasmalogen (PlsEtn) is a subtype of ethanolamine glycerophospholipids (EtnGpl). Recently, PlsEtn has attracted increasing research interest due to its beneficial effects in health and disease; however, its functional role in colonic health has not been well established. This study was conducted to determine the mechanism underlying the antiapoptotic effect of PlsEtn in human intestinal tract cells under induced inflammatory stress. Lipopolysaccharide induced apoptosis of differentiated Caco-2 cells, which was suppressed by EtnGpl in a dose-dependent manner. Cells treated with ascidian muscle EtnGpl containing high levels of PlsEtn demonstrated a lower degree of apoptosis, and downregulated TNF-α and apoptosis-related proteins compared to those treated with porcine liver EtnGpl containing low PlsEtn. This indicates that PlsEtn exerted the observed effects, which provided protection against induced inflammatory stress. Overall, our results suggest that PlsEtn with abundant vinyl ether linkages is potentially beneficial in preventing the initiation of inflammatory bowel disease and colon cancer.
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U2 - 10.1021/acsomega.0c05545
DO - 10.1021/acsomega.0c05545
M3 - Article
AN - SCOPUS:85100233083
VL - 6
SP - 3140
EP - 3148
JO - ACS Omega
JF - ACS Omega
SN - 2470-1343
IS - 4
ER -