TY - JOUR
T1 - Estrogen synthesis in relation to gonadal development of Japanese scallop, Patinopecten yessoensis
T2 - Gonadal profile and immunolocalization of P450 aromatase and estrogen
AU - Osada, Makoto
AU - Tawarayama, Hiroshi
AU - Mori, Katsuyoshi
N1 - Funding Information:
The authors are grateful to the staff of the Field Science Center of Tohoku University for their help with our experiment and to Dr. Yoshio Osawa, Hauptman-Woodward Medical Research Institute, Buffalo, USA, for readily providing us a rabbit anti-human placental aromatase serum. We thank Dr. Mitsuyo Kishida, Kumamoto University, and anonymous reviewers for critical reading. We would appreciate helpful support from Dr. Michio Ohmori, Tohoku University on statistic analysis. This work was supported in part by Grant-in-Aid for Scientific Research (07660231 and 1066071) from the Japanese Ministry of Education, Culture, Sports, Science and Technology (M. Osada).
PY - 2004/9
Y1 - 2004/9
N2 - Aromatase activities and estrogen contents in the gonad of Japanese scallop, Patinopecten yessoensis, were determined during gonadal development and estrogenic cells in the testis were identified immunohistochemically. Ovaries and testes developed rapidly during January and February to reach the mature stage in March and the spawning stage in April. Increases in aromatase activities of the ovary and testis preceded the onset of the ovarian and testicular development. Aromatase activities reached the highest level at the growing stage in February and the mature stage in March, and showed a striking decrease at the spawning stage in April. Contents of ovarian and testicular estradiol-17β changed similarly to the profile of aromatase activities in the ovary and testis, although estrone showed no change. Immunoreactivities against P450 aromatase and estradiol-17β were detected in the cells along the inside of the acinar wall of the testis, whereas in the previous reports, the cells are distributed along the outside of the acinar wall in the ovary. This study thus suggests that estrogen is synthesized in the estrogenic cells of the ovary and testis through aromatization by P450 aromatase and that testicular estrogen may play a physiological role in spermatogenesis.
AB - Aromatase activities and estrogen contents in the gonad of Japanese scallop, Patinopecten yessoensis, were determined during gonadal development and estrogenic cells in the testis were identified immunohistochemically. Ovaries and testes developed rapidly during January and February to reach the mature stage in March and the spawning stage in April. Increases in aromatase activities of the ovary and testis preceded the onset of the ovarian and testicular development. Aromatase activities reached the highest level at the growing stage in February and the mature stage in March, and showed a striking decrease at the spawning stage in April. Contents of ovarian and testicular estradiol-17β changed similarly to the profile of aromatase activities in the ovary and testis, although estrone showed no change. Immunoreactivities against P450 aromatase and estradiol-17β were detected in the cells along the inside of the acinar wall of the testis, whereas in the previous reports, the cells are distributed along the outside of the acinar wall in the ovary. This study thus suggests that estrogen is synthesized in the estrogenic cells of the ovary and testis through aromatization by P450 aromatase and that testicular estrogen may play a physiological role in spermatogenesis.
KW - Estrogen
KW - Gonadal development
KW - HPLC
KW - Immunohistochemistry
KW - P450 aromatase
KW - Scallop
KW - Spermatogenesis
KW - Vitellogenesis
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U2 - 10.1016/j.cbpc.2004.07.002
DO - 10.1016/j.cbpc.2004.07.002
M3 - Article
C2 - 15364295
AN - SCOPUS:4444284272
VL - 139
SP - 123
EP - 128
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 1
ER -