Estimation of the cyclic electron flux around photosystem I in leaf discs

Jiancun Kou, Duncan Fitzpatrick, Da Yong Fan, Shunichi Takahashi, Riichi Oguchi, Wah Soon Chow

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Quantification of the cyclic electron flux (CEF) around photosystem I (PSI) in leaves has been hampered by the absence of net product formation or net substrate consumption. We estimate CEF as the difference between the total electron flux through PSI and the linear electron flux through both photosystems, both fluxes measured in the same leaf tissue in identical conditions. This method can be applied to leaves of plants such as spinach grown in high light and in which the Mehler reaction and charge recombination are minimal. However, leaves of plants such as Arabidopsis grown in low light but tested in high light may have a large electron flux component that cycles within the PSI reaction centre by charge separation followed by charge recombination. In such leaves, CEF can be estimated as the antimycin A-sensitive component of the total electron flux through PSI. However, if CEF is significantly mediated by the nicotinamide adenine dinucleotide dehydrogenase-like complex (NDH), inhibition with a combination of antimycin A and an inhibitor of NDH would be necessary. The method has the potential to be fine-tuned to give a closer estimation of the actual CEF.

Original languageEnglish
Title of host publicationPhotosynthesis and Bioenergetics
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages265-276
Number of pages12
ISBN (Electronic)9789813230309
ISBN (Print)9789813230293
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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  • Cite this

    Kou, J., Fitzpatrick, D., Fan, D. Y., Takahashi, S., Oguchi, R., & Chow, W. S. (2017). Estimation of the cyclic electron flux around photosystem I in leaf discs. In Photosynthesis and Bioenergetics (pp. 265-276). World Scientific Publishing Co. Pte Ltd. https://doi.org/10.1142/9789813230309_0012