Estimation of oxygen metabolism in a rat model of permanent ischemia using positron emission tomography with injectable15O-O2

Takashi Temma, Yasuhiro Magata, Yuji Kuge, Sayaka Shimonaka, Kohei Sano, Yumiko Katada, Hidekazu Kawashima, Takahiro Mukai, Hiroshi Watabe, Hidehiro Iida, Hideo Saji

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The threshold of cerebral blood flow (CBF) into infarction in rats has been indicated to be similar to that in patients. However, CBF does not reflect metabolic function, and so estimations of oxygen metabolism have been required. Here, we estimated changes in oxygen metabolism after occluding the right middle cerebral artery (MCA) in rats using an injectable 15O-O2 we developed. A decrease in CBF (left: 0.67±0.22 mL/min/g, right: 0.44±0.17 mL/min/g, P<0.05) and compensatory increase in the oxygen extraction fraction (OEF) (left: 0.42±0.13, right: 0.50±0.19, P<0.05) were observed at 1-h after occlusion. In contrast, a marked decrease in CBF and the cerebral metabolic rate for oxygen and a collapse of the compensatory OEF mechanism were found at 24 h after occlusion. Injectable 15O-O2 could be used to reliably estimate oxygen metabolism in an infarction rat model with positron emission tomography.

Original languageEnglish
Pages (from-to)1577-1583
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume26
Issue number12
DOIs
Publication statusPublished - 2006 Dec 8

Keywords

  • OEF
  • Oxygen metabolism
  • Permanent ischemia
  • Positron emission tomography
  • Rat

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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