Estimation of interleukin 6 production by reverse transcriptase-polymerase chain reaction in four human myeloma cell lines

Akira Suzuki, Takayuki Takahashi, Yoshiaki Okuno, Manabu Fukumoto, Hiroyasu Fukui, Yasuo Koishihara, Yoshiaki Ohsugi, Yoichioro Ohno, Hiroo Imura

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7 Citations (Scopus)

Abstract

Although an autocrine growth mechanism through interleukin 6 has been advocated in human myeloma cells, reports of IL-6 production by cells from established myeloma cell lines are rare. In the present study, we examined whether or not a minute amount of interleukin 6 is produced in 4 human myeloma cell lines. IL-6 production was not detected in any of the 4 lines by enzyme immunoassay, bioassay with two interleukin 6-dependent murine hybridoma cell lines and Northern hybridization. However, we detected interleukin 6 mRNA in one (U266) of the 4 lines by the reverse transcriptase-polymerase chain reaction. Nevertheless, the proliferation of all 4 lines was not inhibited by an anti-interleukin 6 antibody. These results suggest that autocrine stimulation by interleukin 6 is not involved in the majority of human myeloma cell lines.

Original languageEnglish
Pages (from-to)1043-1050
Number of pages8
JournalLeukemia Research
Volume15
Issue number11
DOIs
Publication statusPublished - 1991

Keywords

  • IL-6
  • Myeloma cell lines
  • RT-PCR

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Suzuki, A., Takahashi, T., Okuno, Y., Fukumoto, M., Fukui, H., Koishihara, Y., Ohsugi, Y., Ohno, Y., & Imura, H. (1991). Estimation of interleukin 6 production by reverse transcriptase-polymerase chain reaction in four human myeloma cell lines. Leukemia Research, 15(11), 1043-1050. https://doi.org/10.1016/0145-2126(91)90110-F