Esterification of xanthophylls by human intestinal Caco-2 cells

Tatsuya Sugawara, Kyoko Yamashita, Akira Asai, Akihiko Nagao, Tomotaka Shiraishi, Ichiro Imai, Takashi Hirata

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We recently found that peridinin, which is uniquely present in dinoflagellates, reduced cell viability by inducing apoptosis in human colon cancer cells. Peridinin is also found in edible clams and oysters because the major food sources of those shellfish are phytoplanktons such as dinoflagellates. Little is known, however, about the fate of dietary peridinin and its biological activities in mammals. The aim of the present study was to investigate the enzymatic esterification of xanthophylls, especially peridinin which is uniquely present in dinoflagellates, using differentiated cultures of Caco-2 human intestinal cells. We found that peridinin is converted to peridininol and its fatty acid esters in differentiated Caco-2 cells treated with 5 μmol/L peridinin solubilized with mixed micelles. The cell homogenate was also able to deacetylate peridinin and to esterify peridininol. Other xanthophylls, such as fucoxanthin, astaxanthin and zeaxanthin, were also esterified, but at relatively lower rates than peridinin. In this study, we found the enzymatic esterification of xanthophylls in mammalian intestinal cells for the first time. Our results suggest that the esterification of xanthophylls in intestinal cells is dependent on their polarity.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume483
Issue number2
DOIs
Publication statusPublished - 2009 Mar 15
Externally publishedYes

Keywords

  • Astaxanthin
  • Carotenoids
  • Fucoxanthin
  • Intestinal cells
  • Peridinin
  • Zeaxanthin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Esterification of xanthophylls by human intestinal Caco-2 cells'. Together they form a unique fingerprint.

Cite this