Abstract
Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.
Original language | English |
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Pages (from-to) | 1169-1171 |
Number of pages | 3 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 82 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2018 |
Keywords
- Gene disruption
- Microbial degradation
- Mycobacterium
- Pah
- Vector plasmid
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry