Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida, Natsumi Ogawa, Eikichi Ichihashi, Hiromi Kato, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

    Original languageEnglish
    Pages (from-to)1169-1171
    Number of pages3
    JournalBioscience, Biotechnology and Biochemistry
    Volume82
    Issue number7
    DOIs
    Publication statusPublished - 2018 Jan 1

    Keywords

    • Gene disruption
    • Microbial degradation
    • Mycobacterium
    • Pah
    • Vector plasmid

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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