Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida; Natsumi Ogawa; Eikichi Ichihashi; Hiromi Kato; Yuji Nagata; Yoshiyuki Ohtsubo; Masataka Tsuda

doi:10.1080/09168451.2018.1445522

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida, Natsumi Ogawa, Eikichi Ichihashi, Hiromi Kato, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

Original language	English
Pages (from-to)	1169-1171
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	82
Issue number	7
DOIs	https://doi.org/10.1080/09168451.2018.1445522
Publication status	Published - 2018

Keywords

Gene disruption
Microbial degradation
Mycobacterium
Pah
Vector plasmid

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2018.1445522

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title = "Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon",

abstract = "Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.",

keywords = "Gene disruption, Microbial degradation, Mycobacterium, Pah, Vector plasmid",

author = "Kouhei Kishida and Natsumi Ogawa and Eikichi Ichihashi and Hiromi Kato and Yuji Nagata and Yoshiyuki Ohtsubo and Masataka Tsuda",

note = "Funding Information: This work was supported by JSPS KAKENHI [grant number 17H03781]. Publisher Copyright: {\textcopyright} 2018 Japan society for Bioscience, Biotechnology, and agrochemistry",

year = "2018",

doi = "10.1080/09168451.2018.1445522",

language = "English",

volume = "82",

pages = "1169--1171",

journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

AU - Kishida, Kouhei

AU - Ogawa, Natsumi

AU - Ichihashi, Eikichi

AU - Kato, Hiromi

AU - Nagata, Yuji

AU - Ohtsubo, Yoshiyuki

AU - Tsuda, Masataka

PY - 2018

Y1 - 2018

N2 - Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

AB - Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

KW - Gene disruption

KW - Microbial degradation

KW - Mycobacterium

KW - Pah

KW - Vector plasmid

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

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ER -

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Abstract

Keywords

ASJC Scopus subject areas

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