Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida, Natsumi Ogawa, Eikichi Ichihashi, Hiromi Kato, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

Original languageEnglish
Pages (from-to)1169-1171
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number7
DOIs
Publication statusPublished - 2018

Keywords

  • Gene disruption
  • Microbial degradation
  • Mycobacterium
  • Pah
  • Vector plasmid

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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