Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida; Natsumi Ogawa; Eikichi Ichihashi; Hiromi Kato; Yuji Nagata; Yoshiyuki Ohtsubo; Masataka Tsuda

doi:10.1080/09168451.2018.1445522

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida, Natsumi Ogawa, Eikichi Ichihashi, Hiromi Kato, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

Original language	English
Pages (from-to)	1169-1171
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	82
Issue number	7
DOIs	https://doi.org/10.1080/09168451.2018.1445522
Publication status	Published - 2018

Keywords

Gene disruption
Microbial degradation
Mycobacterium
Pah
Vector plasmid

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1080/09168451.2018.1445522

Cite this

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon. / Kishida, Kouhei; Ogawa, Natsumi; Ichihashi, Eikichi; Kato, Hiromi ; Nagata, Yuji ; Ohtsubo, Yoshiyuki; Tsuda, Masataka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 7, 2018, p. 1169-1171.

Research output: Contribution to journal › Article › peer-review

Kishida, Kouhei ; Ogawa, Natsumi ; Ichihashi, Eikichi ; Kato, Hiromi ; Nagata, Yuji ; Ohtsubo, Yoshiyuki ; Tsuda, Masataka. / Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon. In: Bioscience, Biotechnology and Biochemistry. 2018 ; Vol. 82, No. 7. pp. 1169-1171.

@article{6632cf3d08de43ada9562a301aee10f0,

title = "Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon",

abstract = "Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.",

keywords = "Gene disruption, Microbial degradation, Mycobacterium, Pah, Vector plasmid",

author = "Kouhei Kishida and Natsumi Ogawa and Eikichi Ichihashi and Hiromi Kato and Yuji Nagata and Yoshiyuki Ohtsubo and Masataka Tsuda",

note = "Funding Information: This work was supported by JSPS KAKENHI [grant number 17H03781]. Publisher Copyright: {\textcopyright} 2018 Japan society for Bioscience, Biotechnology, and agrochemistry",

year = "2018",

doi = "10.1080/09168451.2018.1445522",

language = "English",

volume = "82",

pages = "1169--1171",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "7",

}

TY - JOUR

T1 - Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

AU - Kishida, Kouhei

AU - Ogawa, Natsumi

AU - Ichihashi, Eikichi

AU - Kato, Hiromi

AU - Nagata, Yuji

AU - Ohtsubo, Yoshiyuki

AU - Tsuda, Masataka

PY - 2018

Y1 - 2018

N2 - Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

AB - Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

KW - Gene disruption

KW - Microbial degradation

KW - Mycobacterium

KW - Pah

KW - Vector plasmid

UR - http://www.scopus.com/inward/record.url?scp=85049562150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049562150&partnerID=8YFLogxK

U2 - 10.1080/09168451.2018.1445522

DO - 10.1080/09168451.2018.1445522

M3 - Article

C2 - 29521166

AN - SCOPUS:85049562150

VL - 82

SP - 1169

EP - 1171

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 7

ER -

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this