Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida; Natsumi Ogawa; Eikichi Ichihashi; Hiromi Kato; Yuji Nagata; Yoshiyuki Ohtsubo; Masataka Tsuda

doi:10.1080/09168451.2018.1445522

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Kouhei Kishida, Natsumi Ogawa, Eikichi Ichihashi, Hiromi Kato, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

Original language	English
Pages (from-to)	1169-1171
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	82
Issue number	7
DOIs	https://doi.org/10.1080/09168451.2018.1445522
Publication status	Published - 2018 Jan 1

Keywords

Gene disruption
Microbial degradation
Mycobacterium
Pah
Vector plasmid

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon. / Kishida, Kouhei; Ogawa, Natsumi; Ichihashi, Eikichi; Kato, Hiromi ; Nagata, Yuji ; Ohtsubo, Yoshiyuki; Tsuda, Masataka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 7, 01.01.2018, p. 1169-1171.

Research output: Contribution to journal › Article › peer-review

Kishida, Kouhei ; Ogawa, Natsumi ; Ichihashi, Eikichi ; Kato, Hiromi ; Nagata, Yuji ; Ohtsubo, Yoshiyuki ; Tsuda, Masataka. / Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon. In: Bioscience, Biotechnology and Biochemistry. 2018 ; Vol. 82, No. 7. pp. 1169-1171.

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AU - Nagata, Yuji

AU - Ohtsubo, Yoshiyuki

AU - Tsuda, Masataka

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