Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice

Maiko Kikuchi-Arai; Takashi Murakami; Hiroki Utsunomiya; Jun ichi Akahira; Haruka Suzuki-Kakisaka; Yukihiro Terada; Masahito Tachibana; Shinichi Hayasaka; Tomohisa Ugajin; Nobuo Yaegashi

doi:10.1111/j.1600-0897.2010.00835.x

Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice

Maiko Kikuchi-Arai, Takashi Murakami, Hiroki Utsunomiya, Jun ichi Akahira, Haruka Suzuki-Kakisaka, Yukihiro Terada, Masahito Tachibana, Shinichi Hayasaka, Tomohisa Ugajin, Nobuo Yaegashi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Problem The number of uterus natural killer (NK) cells change through the menstrual cycle, but the origin of uterus NK cell was not unclear. Our aims are to study whether we can reproduce repetition of menstrual cycle and to reveal the origin of uterus NK cells. Method of study Endometrial samples were obtained from fertile women, and the tissues were transplanted into ovariectomized non-obese diabetic (NOD)/severe combined immunodeficiency (SCID)/γCnull (NOG) mice. Mice were treated with sex hormones which were in accord with human menstrual cycle. Results The replants showed similar histological changes as in eutopic endometrium repeatedly. CD56-positive, CD16-negative NK cells increased significantly during the treatment with estradiol and progesterone combination. Conclusion Histological assessment demonstrated that this model of NOG mice repeatedly exhibited regular menstrual cycles, and this model mimicked not 'ectopic endometrium', but 'eutopic endometrium' in humans. Change in number of NK cells suggested that NK cell might be derived from the endometrium.

Original language	English
Pages (from-to)	324-332
Number of pages	9
Journal	American Journal of Reproductive Immunology
Volume	64
Issue number	5
DOIs	https://doi.org/10.1111/j.1600-0897.2010.00835.x
Publication status	Published - 2010 Nov

Keywords

Endometrium
Menstrual cycle
NK cell
NOG mice

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Reproductive Medicine
Obstetrics and Gynaecology

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Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice. / Kikuchi-Arai, Maiko; Murakami, Takashi; Utsunomiya, Hiroki; Akahira, Jun ichi; Suzuki-Kakisaka, Haruka; Terada, Yukihiro; Tachibana, Masahito; Hayasaka, Shinichi; Ugajin, Tomohisa; Yaegashi, Nobuo.

In: American Journal of Reproductive Immunology, Vol. 64, No. 5, 11.2010, p. 324-332.

Research output: Contribution to journal › Article › peer-review

Kikuchi-Arai, Maiko ; Murakami, Takashi ; Utsunomiya, Hiroki ; Akahira, Jun ichi ; Suzuki-Kakisaka, Haruka ; Terada, Yukihiro ; Tachibana, Masahito ; Hayasaka, Shinichi ; Ugajin, Tomohisa ; Yaegashi, Nobuo. / Establishment of Long-Term Model throughout Regular Menstrual Cycles in Immunodeficient Mice. In: American Journal of Reproductive Immunology. 2010 ; Vol. 64, No. 5. pp. 324-332.

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abstract = "Problem The number of uterus natural killer (NK) cells change through the menstrual cycle, but the origin of uterus NK cell was not unclear. Our aims are to study whether we can reproduce repetition of menstrual cycle and to reveal the origin of uterus NK cells. Method of study Endometrial samples were obtained from fertile women, and the tissues were transplanted into ovariectomized non-obese diabetic (NOD)/severe combined immunodeficiency (SCID)/γCnull (NOG) mice. Mice were treated with sex hormones which were in accord with human menstrual cycle. Results The replants showed similar histological changes as in eutopic endometrium repeatedly. CD56-positive, CD16-negative NK cells increased significantly during the treatment with estradiol and progesterone combination. Conclusion Histological assessment demonstrated that this model of NOG mice repeatedly exhibited regular menstrual cycles, and this model mimicked not 'ectopic endometrium', but 'eutopic endometrium' in humans. Change in number of NK cells suggested that NK cell might be derived from the endometrium.",

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author = "Maiko Kikuchi-Arai and Takashi Murakami and Hiroki Utsunomiya and Akahira, {Jun ichi} and Haruka Suzuki-Kakisaka and Yukihiro Terada and Masahito Tachibana and Shinichi Hayasaka and Tomohisa Ugajin and Nobuo Yaegashi",

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AU - Suzuki-Kakisaka, Haruka

AU - Terada, Yukihiro

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AU - Ugajin, Tomohisa

AU - Yaegashi, Nobuo

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N2 - Problem The number of uterus natural killer (NK) cells change through the menstrual cycle, but the origin of uterus NK cell was not unclear. Our aims are to study whether we can reproduce repetition of menstrual cycle and to reveal the origin of uterus NK cells. Method of study Endometrial samples were obtained from fertile women, and the tissues were transplanted into ovariectomized non-obese diabetic (NOD)/severe combined immunodeficiency (SCID)/γCnull (NOG) mice. Mice were treated with sex hormones which were in accord with human menstrual cycle. Results The replants showed similar histological changes as in eutopic endometrium repeatedly. CD56-positive, CD16-negative NK cells increased significantly during the treatment with estradiol and progesterone combination. Conclusion Histological assessment demonstrated that this model of NOG mice repeatedly exhibited regular menstrual cycles, and this model mimicked not 'ectopic endometrium', but 'eutopic endometrium' in humans. Change in number of NK cells suggested that NK cell might be derived from the endometrium.

AB - Problem The number of uterus natural killer (NK) cells change through the menstrual cycle, but the origin of uterus NK cell was not unclear. Our aims are to study whether we can reproduce repetition of menstrual cycle and to reveal the origin of uterus NK cells. Method of study Endometrial samples were obtained from fertile women, and the tissues were transplanted into ovariectomized non-obese diabetic (NOD)/severe combined immunodeficiency (SCID)/γCnull (NOG) mice. Mice were treated with sex hormones which were in accord with human menstrual cycle. Results The replants showed similar histological changes as in eutopic endometrium repeatedly. CD56-positive, CD16-negative NK cells increased significantly during the treatment with estradiol and progesterone combination. Conclusion Histological assessment demonstrated that this model of NOG mice repeatedly exhibited regular menstrual cycles, and this model mimicked not 'ectopic endometrium', but 'eutopic endometrium' in humans. Change in number of NK cells suggested that NK cell might be derived from the endometrium.

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