Establishment of evaluation method to determine effects of veterinary medicinal products on manure fermentation using small-scale composting apparatus

Kaoru Eguchi, Kenichi Otawa, Ryu Ohishi, Hiroyasu Nagase, Tomoko Ogata, Hidetaka Nagai, Nanae Murata, Hitoshi Ishikawa, Kazumasa Hirata, Yutaka Nakai

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

To evaluate on a laboratory scale the influence of veterinary medicinal products (VMPs) excreted into feces on manure fermentation, we have developed an evaluation method that uses a small-scale composting apparatus. Each run is of approximately 3kg scale and the operation can be conducted in an environmentally controlled laboratory. The main evaluation parameter is calorific value generated by aerobic fermentation. At the sulfadimethoxine (SDM) trial, the volume of CO2 generated during fermentation and the disappearance of the inhibitory effect of immature manure on sprouting (using Komatsuna (Brassica rapa var. perviridis)) were measured. In addition, DNA of 16S rRNA was extracted from a manure sample and subjected to denaturing gradient gel electrophoresis (DGGE). The results suggest that the presence of such VMPs in feces affected the microbial community in manure fermentation, and indicate that the evaluation method may be used as a standard method to evaluate the effect of VMPs on the microbial community. Using the method, we obtained data of the influence of five VMPs approved for stockbreeding in Japan on swine manure fermentation. Erythromycin (EM) affected the calorific value even at a relatively low concentration (105mg/3kg manure). In contrast, oxytetracycline hydrochloride (OTC), norfloxacin (NFLX), and tylosin tartrate (TS) had no effect at that concentration. These VMPs also affected the increase of fermentation temperature when added at high concentrations.

Original languageEnglish
Pages (from-to)312-317
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume114
Issue number3
DOIs
Publication statusPublished - 2012 Sep 1

Keywords

  • Ecotoxicity
  • Fermentation
  • Manure
  • Pharmaceutics
  • Stockbreeding

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Establishment of evaluation method to determine effects of veterinary medicinal products on manure fermentation using small-scale composting apparatus'. Together they form a unique fingerprint.

Cite this