TY - JOUR
T1 - Establishment of evaluation method to determine effects of veterinary medicinal products on manure fermentation using small-scale composting apparatus
AU - Eguchi, Kaoru
AU - Otawa, Kenichi
AU - Ohishi, Ryu
AU - Nagase, Hiroyasu
AU - Ogata, Tomoko
AU - Nagai, Hidetaka
AU - Murata, Nanae
AU - Ishikawa, Hitoshi
AU - Hirata, Kazumasa
AU - Nakai, Yutaka
PY - 2012/9/1
Y1 - 2012/9/1
N2 - To evaluate on a laboratory scale the influence of veterinary medicinal products (VMPs) excreted into feces on manure fermentation, we have developed an evaluation method that uses a small-scale composting apparatus. Each run is of approximately 3kg scale and the operation can be conducted in an environmentally controlled laboratory. The main evaluation parameter is calorific value generated by aerobic fermentation. At the sulfadimethoxine (SDM) trial, the volume of CO2 generated during fermentation and the disappearance of the inhibitory effect of immature manure on sprouting (using Komatsuna (Brassica rapa var. perviridis)) were measured. In addition, DNA of 16S rRNA was extracted from a manure sample and subjected to denaturing gradient gel electrophoresis (DGGE). The results suggest that the presence of such VMPs in feces affected the microbial community in manure fermentation, and indicate that the evaluation method may be used as a standard method to evaluate the effect of VMPs on the microbial community. Using the method, we obtained data of the influence of five VMPs approved for stockbreeding in Japan on swine manure fermentation. Erythromycin (EM) affected the calorific value even at a relatively low concentration (105mg/3kg manure). In contrast, oxytetracycline hydrochloride (OTC), norfloxacin (NFLX), and tylosin tartrate (TS) had no effect at that concentration. These VMPs also affected the increase of fermentation temperature when added at high concentrations.
AB - To evaluate on a laboratory scale the influence of veterinary medicinal products (VMPs) excreted into feces on manure fermentation, we have developed an evaluation method that uses a small-scale composting apparatus. Each run is of approximately 3kg scale and the operation can be conducted in an environmentally controlled laboratory. The main evaluation parameter is calorific value generated by aerobic fermentation. At the sulfadimethoxine (SDM) trial, the volume of CO2 generated during fermentation and the disappearance of the inhibitory effect of immature manure on sprouting (using Komatsuna (Brassica rapa var. perviridis)) were measured. In addition, DNA of 16S rRNA was extracted from a manure sample and subjected to denaturing gradient gel electrophoresis (DGGE). The results suggest that the presence of such VMPs in feces affected the microbial community in manure fermentation, and indicate that the evaluation method may be used as a standard method to evaluate the effect of VMPs on the microbial community. Using the method, we obtained data of the influence of five VMPs approved for stockbreeding in Japan on swine manure fermentation. Erythromycin (EM) affected the calorific value even at a relatively low concentration (105mg/3kg manure). In contrast, oxytetracycline hydrochloride (OTC), norfloxacin (NFLX), and tylosin tartrate (TS) had no effect at that concentration. These VMPs also affected the increase of fermentation temperature when added at high concentrations.
KW - Ecotoxicity
KW - Fermentation
KW - Manure
KW - Pharmaceutics
KW - Stockbreeding
UR - http://www.scopus.com/inward/record.url?scp=84864605493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864605493&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2012.04.001
DO - 10.1016/j.jbiosc.2012.04.001
M3 - Article
C2 - 22595343
AN - SCOPUS:84864605493
VL - 114
SP - 312
EP - 317
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -