Establishment of C20Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells

Yoshikazu Furusawa, Mika Kato Kaneko, Yukinari Kato

Research output: Contribution to journalArticle

Abstract

CD20 is one of several B-lymphocyte antigens that has been shown to be an effective target for the detection and treatment of B-cell lymphomas. Sensitive and specific monoclonal antibodies (mAbs) are required for every application used for the diagnosis of B-cell lymphoma. Although many anti-CD20 mAbs have been established, the types of applications, those anti-CD20 can be used in, are limited. In this study, we aimed to establish novel anti-CD20 mAbs to be used for broad applications, such as flow cytometry, western blot, and immunohistochemical analyses, using the Cell-Based Immunization and Screening (CBIS) method. One of the established mAbs, C20Mab-11 (IgM, kappa), detected overexpression of CD20 in CHO-K1 or LN229 cell lines, indicating that C20Mab-11 is specific for CD20. In western blot analyses, C20Mab-11 detected not only overexpression of CD20 in CHO-K1 or LN229 cell lines, but also CD20 of BALL-1 and Raji cells with both sensitivity and specificity. Furthermore, C20Mab-11 strongly stained B cells of the lymph follicle and B cell lymphomas in immunohistochemical analyses. These results indicate that C20Mab-11 develped by CBIS method, is useful for the detection of CD20 in lymphoma tissues by flow cytometry, western blot, and immunohistochemical analyses and potentially could be beneficial for the treatment of B cell lymphomas.

Original languageEnglish
Pages (from-to)1961-1967
Number of pages7
JournalOncology Letters
Volume20
Issue number2
DOIs
Publication statusPublished - 2020 Aug

Keywords

  • CD20
  • Flow cytometry
  • Immunohistochemistry
  • Monoclonal antibody
  • Western blotting

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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