Establishment of cell lines derived from the genus macaca through controlled expression of cell cycle regulators

Kengo Kuroda, Tohru Kiyono, Takahiro Eitsuka, Hiroshi Isogai, Koichi Takahashi, Kenichiro Donai, Emiko Isogai, Tomokazu Fukuda

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Nonhuman primates are useful animal models for the study of human diseases. However, the number of established cell lines from nonhuman primates is quite limited compared with the number established from other experimental animals. The establishment of nonhuman primate cell lines would allow drug testing on those cell lines before moving experiments into primates. In this study, we established nonhuman primate primary cell lines by introducing the genes for CDK4R24C, cyclin D1, and hTERT. These cell lines proliferated more rapidly than primary cells and bypassed cellular senescence. Karyotype analysis showed that the chromosome patterns were intact in the immortalized cell lines. Furthermore, we showed that the expression of introduced genes could be precisely controlled through the Tet-Off system with the addition of doxycycline. The present study shows that introduction of the CDK4R24C, cyclin D1, and hTERT genes are effective methods of establishing nonhuman primate cell lines.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalJournal of Cellular Biochemistry
Volume116
Issue number2
DOIs
Publication statusPublished - 2015 Feb

Keywords

  • CDK4R24C
  • Cyclin D1
  • Macaque Monkey
  • Nonhuman Primate Model
  • hTert

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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