Establishment of An Estrogen Receptor-Positive Cell Line (HMA-1) Derived from Human Breast Carcinoma

Shigeo Matano, Noriaki Ohuchi, Hisashi Hirakawa, Tetsuro Nishihira, Motoi Abe, Shozo Mori, Minoru Akimoto

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


We have established a novel human breast carcinoma cell line, HMA-1, derived from ascites of a female breast cancer patient. HMA-1 was shown to be an epithelial cell line with intracytoplasmic duct-like vacuoles, microvilli, desmosomes and tonofibrils in accordance with human breast cancer. The cell line demonstrated a good cell growth ability in monolayer fashion with a doubling time of 46 hr. Based on a whole cell binding assay the cell line contained estrogen receptor (1.45 × 10-4 sites/cell). Tamoxifen, an anti-estrogen agent induced a dose-dependent decrease in the cell growth rate, but estradiol stimulated the cell growth. HMA-1 could be transplanted subcutaneously into BALB/c nude mice, and was able to cause tumors approximately two months after heteroinoculation. These results indicate that HMA-1 cell line may serve as a new human breast carcinoma cell line which could be utilized in the breast cancer research.

Original languageEnglish
Pages (from-to)169-182
Number of pages14
JournalTohoku Journal of Experimental Medicine
Issue number2
Publication statusPublished - 1991


  • breast neoplasm
  • estrogen receptor
  • human cell line

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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