Establishment of an Anticetacean Podoplanin Monoclonal Antibody PMab-237 for Immunohistochemical Analysis

Yukinari Kato, Yoshikazu Furusawa, Shunsuke Itai, Junko Takei, Takuro Nakamura, Masato Sano, Hiroyuki Harada, Shinji Yamada, Mika K. Kaneko

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Podoplanin (PDPN) has been utilized as a lymphatic endothelial cell marker especially in pathological diagnoses. Therefore, sensitive and specific monoclonal antibodies (mAbs) targeting PDPN are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues. Recently, anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, and horse were established in our studies. However, anticetacean (whale) PDPN (wPDPN) has not been established yet. In this study, we immunized mice with wPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/wPDPN) cells, and screened mAbs against wPDPN using flow cytometry. One of the mAbs, PMab-237 (IgG1, kappa), specifically detected CHO/wPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-237 for the functional analyses of wPDPN.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number3
DOIs
Publication statusPublished - 2019 Jun

Keywords

  • PDPN
  • PMab-237
  • lymphatic endothelial cells
  • whale podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Establishment of an Anticetacean Podoplanin Monoclonal Antibody PMab-237 for Immunohistochemical Analysis'. Together they form a unique fingerprint.

  • Cite this