Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo

Hitoshi Yamazaki, Yoshiyuki Abe, Yuko Katoh, Nobuko Sawa, Yasuyuki Ohnishi, Yuji Tanaka, Hironobu Sasano, Yoshiro Oshika, Testuji Tokunaga, Hiroshi Kijima, Norikazu Tamaoki, Masato Nakamura, Yoshito Ueyama

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We established a xenograft line of human adrenocortical carcinoma (ADR- 1), and analyzed the hyperaldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25-year-old woman were subcutaneously inoculated into nude mice (BALB/c-nu/nu) followed by serial passages in vivo. ADR-1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/l), but did not show hypertension. The nude rat (F344-rnu/rnu) bearing ADR-1 showed hyperaldosteronism (serum aldosterone 3320±1420 pg/ml; control 191±130 pg/ml) and hypokalemia (serum K 3.4+0.4 mEq/1; control 5.2±1.0 mEq/1) in vivo, and hypertension was not obvious. ADR-1 was shown immunohistochemically to retain production of human-specific corticosteroid synthetase. The xenograft ADR-1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.

Original languageEnglish
Pages (from-to)1056-1060
Number of pages5
JournalAPMIS
Volume106
Issue number11
DOIs
Publication statusPublished - 1998

Keywords

  • Adrenocortical carcinoma
  • Aldosterone
  • Xenograft

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Microbiology (medical)

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