Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo

Hitoshi Yamazaki; Yoshiyuki Abe; Yuko Katoh; Nobuko Sawa; Yasuyuki Ohnishi; Yuji Tanaka; Hironobu Sasano; Yoshiro Oshika; Testuji Tokunaga; Hiroshi Kijima; Norikazu Tamaoki; Masato Nakamura; Yoshito Ueyama

doi:10.1111/j.1699-0463.1998.tb00258.x

Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo

Hitoshi Yamazaki, Yoshiyuki Abe, Yuko Katoh, Nobuko Sawa, Yasuyuki Ohnishi, Yuji Tanaka, Hironobu Sasano, Yoshiro Oshika, Testuji Tokunaga, Hiroshi Kijima, Norikazu Tamaoki, Masato Nakamura, Yoshito Ueyama

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We established a xenograft line of human adrenocortical carcinoma (ADR- 1), and analyzed the hyperaldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25-year-old woman were subcutaneously inoculated into nude mice (BALB/c-nu/nu) followed by serial passages in vivo. ADR-1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/l), but did not show hypertension. The nude rat (F344-rnu/rnu) bearing ADR-1 showed hyperaldosteronism (serum aldosterone 3320±1420 pg/ml; control 191±130 pg/ml) and hypokalemia (serum K 3.4+0.4 mEq/1; control 5.2±1.0 mEq/1) in vivo, and hypertension was not obvious. ADR-1 was shown immunohistochemically to retain production of human-specific corticosteroid synthetase. The xenograft ADR-1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.

Original language	English
Pages (from-to)	1056-1060
Number of pages	5
Journal	APMIS
Volume	106
Issue number	11
DOIs	https://doi.org/10.1111/j.1699-0463.1998.tb00258.x
Publication status	Published - 1998

Keywords

Adrenocortical carcinoma
Aldosterone
Xenograft

ASJC Scopus subject areas

Pathology and Forensic Medicine
Immunology and Allergy
Microbiology (medical)

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Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo. / Yamazaki, Hitoshi; Abe, Yoshiyuki; Katoh, Yuko; Sawa, Nobuko; Ohnishi, Yasuyuki; Tanaka, Yuji; Sasano, Hironobu; Oshika, Yoshiro; Tokunaga, Testuji; Kijima, Hiroshi; Tamaoki, Norikazu; Nakamura, Masato; Ueyama, Yoshito.

In: APMIS, Vol. 106, No. 11, 1998, p. 1056-1060.

Research output: Contribution to journal › Article › peer-review

Yamazaki, Hitoshi ; Abe, Yoshiyuki ; Katoh, Yuko ; Sawa, Nobuko ; Ohnishi, Yasuyuki ; Tanaka, Yuji ; Sasano, Hironobu ; Oshika, Yoshiro ; Tokunaga, Testuji ; Kijima, Hiroshi ; Tamaoki, Norikazu ; Nakamura, Masato ; Ueyama, Yoshito. / Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo. In: APMIS. 1998 ; Vol. 106, No. 11. pp. 1056-1060.

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abstract = "We established a xenograft line of human adrenocortical carcinoma (ADR- 1), and analyzed the hyperaldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25-year-old woman were subcutaneously inoculated into nude mice (BALB/c-nu/nu) followed by serial passages in vivo. ADR-1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/l), but did not show hypertension. The nude rat (F344-rnu/rnu) bearing ADR-1 showed hyperaldosteronism (serum aldosterone 3320±1420 pg/ml; control 191±130 pg/ml) and hypokalemia (serum K 3.4+0.4 mEq/1; control 5.2±1.0 mEq/1) in vivo, and hypertension was not obvious. ADR-1 was shown immunohistochemically to retain production of human-specific corticosteroid synthetase. The xenograft ADR-1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.",

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