Abstract
We established a xenograft line of human adrenocortical carcinoma (ADR- 1), and analyzed the hyperaldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25-year-old woman were subcutaneously inoculated into nude mice (BALB/c-nu/nu) followed by serial passages in vivo. ADR-1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/l), but did not show hypertension. The nude rat (F344-rnu/rnu) bearing ADR-1 showed hyperaldosteronism (serum aldosterone 3320±1420 pg/ml; control 191±130 pg/ml) and hypokalemia (serum K 3.4+0.4 mEq/1; control 5.2±1.0 mEq/1) in vivo, and hypertension was not obvious. ADR-1 was shown immunohistochemically to retain production of human-specific corticosteroid synthetase. The xenograft ADR-1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.
Original language | English |
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Pages (from-to) | 1056-1060 |
Number of pages | 5 |
Journal | APMIS |
Volume | 106 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1998 |
Externally published | Yes |
Keywords
- Adrenocortical carcinoma
- Aldosterone
- Xenograft
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Immunology and Allergy
- Microbiology (medical)