Establishment of a novel cell line (TS-2) of pre-B acute lymphoblastic leukemia with a T(1;19) not involving the E2A gene

Miyako Yoshinari, Masue Imaizumi, Mariko Eguchi, Masahito Ogasawara, Toshiaki Saito, Hoshiro Suzuki, Yoshitsugu Koizumi, Yan Cui, Atsushi Sato, Takako Saisho, Ryo Ichinohasama, Yoichi Matsubara, Nanao Kamada, Kazuie Iinuma

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The t(1;19)(q23;p13) translocation involving the E2A gene on chromosome 19p13.3 is a nonrandom translocation that is often seen in childhood pre-B- cell acute lymphoblastic leukemia (ALL). However, recent studies have demonstrated the presence of immunophenotypic and molecular heterogeneity among patients with the cytogenetically identical chromosome translocation. Here we report a novel pre-B ALL cell line, TS-2, with t(1;19) translocation not involving the E2A gene. The breakpoint of t(1;19) in TS-2 was demonstrated to be at 19p13.3, a region indistinguishable from the locus of the E2A gene, by cytogenetic study and fluorescence in situ hybridization. However, rearrangement of the E2A gene was not detected in TS-2 by Southern blot analysis. Moreover, the expressions of PBX1 or E2A/PBX1 fusion genes were not detected by an extensive study with Northern blot analysis and reverse transcription-polymerase chain reaction. These findings suggest that TS-2 may have a genetic abnormality involving uncharacterized gene(s) at 19p13.3 distinct from the E2A gene and, therefore, may be useful for investigating the heterogeneity of molecular pathogenesis in leukemias with t(1;19)(q23;p13) translocation.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalCancer Genetics and Cytogenetics
Volume101
Issue number2
DOIs
Publication statusPublished - 1998 Mar

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Establishment of a novel cell line (TS-2) of pre-B acute lymphoblastic leukemia with a T(1;19) not involving the E2A gene'. Together they form a unique fingerprint.

Cite this