Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1

Susumu Saijyo, Toshio Kudo, Masanori Suzuki, Yu Katayose, Masao Shinoda, Taisei Muto, Kenji Fukuhara, Takao Suzuki, Seiki Matsuno

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 colon of K-ras was detected, expression of c-erbB-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalTohoku Journal of Experimental Medicine
Volume177
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1

Keywords

  • MUC1
  • TFK-1
  • c-erbB-2
  • cell line
  • extrahepatic bile duct carcinoma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1'. Together they form a unique fingerprint.

  • Cite this

    Saijyo, S., Kudo, T., Suzuki, M., Katayose, Y., Shinoda, M., Muto, T., Fukuhara, K., Suzuki, T., & Matsuno, S. (1995). Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1. Tohoku Journal of Experimental Medicine, 177(1), 61-71. https://doi.org/10.1620/tjem.177.61