Establishment of a monoclonal antibody PMab-233 for immunohistochemical analysis against Tasmanian devil podoplanin

Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Junko Takei, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Monoclonal antibodies (mAbs) against not only human, mouse, and rat but also rabbit, dog, cat, bovine, pig, and horse podoplanins (PDPNs) have been established in our previous studies. PDPN is used as a lymphatic endothelial cell marker in pathological diagnoses. However, mAbs against Tasmanian devil PDPN (tasPDPN), which are useful for immunohistochemical analysis, remain to be developed. Herein, mice were immunized with tasPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/tasPDPN) cells, and hybridomas producing mAbs against tasPDPN were screened using flow cytometry. One of the mAbs, PMab-233 (IgG1, kappa), specifically detected CHO/tasPDPN cells by flow cytometry and recognized tasPDPN protein by western blotting. Furthermore, PMab-233 strongly detected CHO/tasPDPN cells by immunohistochemistry. These findings suggest that PMab-233 may be useful as a lymphatic endothelial cell marker of the Tasmanian devil.

Original languageEnglish
Article number100631
JournalBiochemistry and Biophysics Reports
Volume18
DOIs
Publication statusPublished - 2019 Jul

Keywords

  • PDPN
  • PMab-233
  • Tasmanian devil podoplanin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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