Establishment of a feeder-cell-dependent cd4+ cd8+ t cell line, l-kim, from a patient with non-hodgkin's lymphoma

H. Fuiie, S. Tsuchiya, M. Minegishi, Y. Sasahara, S. Kumaki, M. Itano, S. Morita, S. Kawai, T. Konno

Research output: Contribution to journalArticle

Abstract

Thymic T cell lymphoma cell line, L-KIM, was established from pleural fluid of a 13-year-old female patient with nonHodgkin's lymphoma at the first relapse. The lymphorna cells were originally proliferated only on the fibroblast-like cells transferred from her pleural fluid and finally adapted to grow on the irradiated Balb3T3 cells. Human fibroblast cell lines, HEL-O and MRC5, and mouse NIH3T3 cells also support the growth of L-KIM, but the cells never grew without those feeder cells. L-KIM did not adhere nor grow on the fibronectin-, laminin- and collagen type II-coated dishes. Culture supernatants of Balb/3T3 cells showed no activity on the proliferation of M-MOK cells. Chromosome analyses revealed 46XX, t (7;9) (q32;q34), invl4(qllq24). Cell surface antigen analyses showed that L-KIM was positive for CD1, CD2, CD3, CD4, CD8.CD21, CD41L and CD95. CD3 positive cells were separated into 2 populations, majority was low in its expression and less than 10% of the cells high with TCR a expression. Thus L-KIM seemed to be a counterpart of CD4 CDS double positive thymic T cells that were in the stage of positive and negative selection. Stimulation by phytohemaggulutinin induced transient growth of the cells, but could not sustain the long term in vitro growth even in the presence of IL-2. The L-KIM cell line may be of value in studying interacting mechanisms between stromal cells and thymocytes and molecular events of differentiation in CD3 low, double positive thymocytes.

Original languageEnglish
Number of pages1
JournalExperimental Hematology
Volume25
Issue number8
Publication statusPublished - 1997 Dec 1

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Fuiie, H., Tsuchiya, S., Minegishi, M., Sasahara, Y., Kumaki, S., Itano, M., Morita, S., Kawai, S., & Konno, T. (1997). Establishment of a feeder-cell-dependent cd4+ cd8+ t cell line, l-kim, from a patient with non-hodgkin's lymphoma. Experimental Hematology, 25(8).