Establishment and functional characterization of novel natural killer cell lines derived from a temperature-sensitive SV40 large T antigen transgenic mouse

Satoru Iizuka, Tomonori Kaifu, Akira Nakamura, Masuo Obinata, Toshiyuki Takai

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Natural killer (NK) cells belong to an important lymphocyte population that eliminates transformed cells and invading pathogens without any prior sensitization. NK cells possess not only natural killing activity against non-self and altered-self cells but also exhibit cytokine production and antibody-dependent cell-mediated cytotoxicity (ADCC). Despite their important roles in the innate immune system, little is known about the details of NK cell biology. In spite of that several murine NK cell clones have been established, studies have mainly focused on their natural killing activity but not their cytokine production or ADCC. In this study, we established and characterized eight novel, immortalized murine NK cell clones derived from a temperature-sensitive SV40 large-T antigen transgenic mouse. These NK cell lines continuously proliferated for more than 30 months in a culture medium supplemented with interleukin 2. All cell lines contained azurophilic granules in the cytoplasm, and a few clones retained the NK cell functions, such as natural killing activity, cytokine production, and ADCC. In addition, one clone could serve as a host for transient as well as stable gene transfection. Taken together, these findings indicate that the cell lines could constitute useful tools for detailed analysis of murine NK cell biology.

Original languageEnglish
Pages (from-to)255-265
Number of pages11
JournalJournal of biochemistry
Volume140
Issue number2
DOIs
Publication statusPublished - 2006 Aug 1

Keywords

  • Cell-culture
  • Cell-differentiation
  • Cell-sorting
  • Immuology-cellular response
  • Receptor-lyeukocyte/lymphocyte

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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