Establishment and Epitope Mapping of Anti-Diacylglycerol Kinase α Monoclonal Antibody DaMab-8 for Immunohistochemical Analyses

Masato Sano, Mika K. Kaneko, Hiroyoshi Suzuki, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). DGKα, 1 of the 10 DGK isozymes, is involved in T cell function. In the present study, we describe a specific monoclonal antibody DaMab-8 (mouse IgG1, kappa) against DGKα, which is extremely useful for performing immunohistochemical analysis for T cells in oropharyngeal squamous cell carcinomas. Furthermore, we characterized the binding epitope of DaMab-8 using Western blotting and found that the sites Asn610, Leu611, Trp612, Gly613, Asp614, His619, Tyr623, and Gly624 of DGKα are important for facilitating the DaMab-8 binding to the DGKα protein. Thus, DaMab-8 could be advantageous for immunohistochemical analyses toward clarifying the distribution of DGKα-expressing T cells in every pathophysiological tissue.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume39
Issue number1
DOIs
Publication statusPublished - 2020 Feb

Keywords

  • DGKα
  • diacylglycerol kinase
  • epitope mapping
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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