Establishment and characterization of CAG/EGFP transgenic rabbit line

Ri Ichi Takahashi, Takashi Kuramochi, Kazuki Aoyagi, Shu Hashimoto, Ichiro Miyoshi, Noriyuki Kasai, Yoji Hakamata, Eiji Kobayashi, Masatsugu Ueda

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Cell marking is a very important procedure for identifying donor cells after cell and/or organ transplantation in vivo. Transgenic animals expressing marker proteins such as enhanced green fluorescent protein (EGFP) in their tissues are a powerful tool for research in fields of tissue engineering and regenerative medicine. The purpose of this study was to establish transgenic rabbit lines that ubiquitously express EGFP under the control of the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG) to provide a fluorescent transgenic animal as a bioresource. We microinjected the EGFP expression vector into 945 rabbit eggs and 4 independent transgenic candidate pups were obtained. Two of them died before sexual maturation and one was infertile. One transgenic male candidate founder rabbit was obtained and could be bred by artificial insemination. The rabbit transmitted the transgene in a Mendelian manner. Using fluorescence in situ hybridization analysis, we detected the transgene at 7q11 on chromosome 7 as a large centromeric region in two F1 offspring (one female and one male). Eventually, one transgenic line was established. Ubiquitous EGFP florescence was confirmed in all examined organs. There were no gender-related differences in fluorescence. The established CAG/EGFP transgenic rabbit will be an important bioresource and a useful tool for various studies in tissue engineering and regenerative medicine.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalTransgenic Research
Volume16
Issue number1
DOIs
Publication statusPublished - 2007 Feb
Externally publishedYes

Keywords

  • Bioresource
  • CAG
  • EGFP
  • Transgenic rabbit

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

Fingerprint Dive into the research topics of 'Establishment and characterization of CAG/EGFP transgenic rabbit line'. Together they form a unique fingerprint.

Cite this