Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line

Akira Nakata, Takashi Kameda, Hirokazu Nagai, Kenji Ikegami, Yuqing Duan, Kunihiko Terada, Toshihiro Sugiyama

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)


Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we tried to establish a spontaneously immortalized ameloblast-lineage cell line, maintaining the cell type specific character, including the ability to induce in vitro bio-mineralization. The established cell line (ameloblast-lineage cell; ALC) maintained the expression of several ameloblast specific genes (Amelogenin, Tuftelin, and Enamelin) in long-term culture. They formed calcified nodules after the induction by medium switching from SMEM to DMEM, having high-level alkaline-phosphatase activity. The size and number of calcified nodule formation were enhanced by TGF-β treatment. Six weeks after sub-cutaneous implantation of ALC to athymic nude mice, we ectopically observed enamel epithelium like structure formation, chondrogenesis, and calcification. These data indicate that ALC is a useful experimental tool to analyze ameloblast character.

Original languageEnglish
Pages (from-to)834-839
Number of pages6
JournalBiochemical and biophysical research communications
Issue number4
Publication statusPublished - 2003 Sep 5
Externally publishedYes


  • Ameloblast
  • Calcification
  • Enamel matrix
  • Spontaneous immortalization

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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