TY - JOUR
T1 - Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line
AU - Nakata, Akira
AU - Kameda, Takashi
AU - Nagai, Hirokazu
AU - Ikegami, Kenji
AU - Duan, Yuqing
AU - Terada, Kunihiko
AU - Sugiyama, Toshihiro
N1 - Funding Information:
This work was supported in part by a grant for the Research for the Future Program from the Japan Society for the Promotion of Science, by Grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2003/9/5
Y1 - 2003/9/5
N2 - Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we tried to establish a spontaneously immortalized ameloblast-lineage cell line, maintaining the cell type specific character, including the ability to induce in vitro bio-mineralization. The established cell line (ameloblast-lineage cell; ALC) maintained the expression of several ameloblast specific genes (Amelogenin, Tuftelin, and Enamelin) in long-term culture. They formed calcified nodules after the induction by medium switching from SMEM to DMEM, having high-level alkaline-phosphatase activity. The size and number of calcified nodule formation were enhanced by TGF-β treatment. Six weeks after sub-cutaneous implantation of ALC to athymic nude mice, we ectopically observed enamel epithelium like structure formation, chondrogenesis, and calcification. These data indicate that ALC is a useful experimental tool to analyze ameloblast character.
AB - Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we tried to establish a spontaneously immortalized ameloblast-lineage cell line, maintaining the cell type specific character, including the ability to induce in vitro bio-mineralization. The established cell line (ameloblast-lineage cell; ALC) maintained the expression of several ameloblast specific genes (Amelogenin, Tuftelin, and Enamelin) in long-term culture. They formed calcified nodules after the induction by medium switching from SMEM to DMEM, having high-level alkaline-phosphatase activity. The size and number of calcified nodule formation were enhanced by TGF-β treatment. Six weeks after sub-cutaneous implantation of ALC to athymic nude mice, we ectopically observed enamel epithelium like structure formation, chondrogenesis, and calcification. These data indicate that ALC is a useful experimental tool to analyze ameloblast character.
KW - Ameloblast
KW - Calcification
KW - Enamel matrix
KW - Spontaneous immortalization
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U2 - 10.1016/S0006-291X(03)01467-0
DO - 10.1016/S0006-291X(03)01467-0
M3 - Article
C2 - 12927794
AN - SCOPUS:0041659208
VL - 308
SP - 834
EP - 839
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -