Establishment and characterization of a novel ovarian clear cell adenocarcinoma cell line, TU-OC-1, with a mutation in the PIK3CA gene

Hiroaki Itamochi, Misaki Kato, Mayumi Nishimura, Nao Oumi, Tetsuro Oishi, Muneaki Shimada, Shinya Sato, Jun Naniwa, Seiya Sato, Michiko Nonaka, Akiko Kudoh, Naoki Terakawa, Junzo Kigawa, Tasuku Harada

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A new cell line of human ovarian clear cell adenocarcinoma (CCC), TU-OC-1, was established and characterized. The cells showed a polygonal-shaped morphology and grew in monolayers without contact inhibition and were arranged like a jigsaw puzzle. The chromosome numbers ranged from 64 to 90. A low rate of proliferation was observed, similar to other CCC cell lines tested (OVTOKO, RMG-I, and OVAS), and the doubling time was 38.4 h. The respective IC50 values of cisplatin and paclitaxel were 12.2 μM and 58.3 nM. Mutational analysis revealed that TU-OC-1 cells harbored a PIK3CA mutation at codon 542 (E542K) in exon 9, which is a mutation hot spot on this gene. We observed that phosphorylated Akt protein was overexpressed in TU-OC-1 cells by western blot analysis. Heterotransplantation to nude mice produced tumors that reflected the original. This cell line may be useful to study the chemoresistant mechanisms of CCC and contribute to novel treatment strategies.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalHuman Cell
Volume26
Issue number3
DOIs
Publication statusPublished - 2013 Sep
Externally publishedYes

Keywords

  • Chemosensitivity
  • Establishment
  • Molecular targeted therapy
  • Ovarian clear cell adenocarcinoma
  • PIK3CA mutation

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

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