Establishment and characterization of a cisplatin-resistant human neuroblastoma cell line

Tetsuya Yasuno, Takafumi Matsumura, Takuma Shikata, Johji Inazawa, Tomoya Sakabe, Shigeki Tsuchida, Takenori Takahata, Shinichi Miyairi, Akira Naganuma, Tadashi Sawada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: To investigate the mechanisms of cisplatin (CDDP)-resistance in neuroblastoma (NB), we established a CDDP-resistant human NB cell line, BM1R2. Materials and Methods: We characterized BM1R2 in terms of the susceptibilities to other anticancer agents, MDR1 and MRP expression, MYCN amplification, intracellular gultathione-S-transrerase (GST-π), metallothionein (MT) and gultathione (GSH) levels, and immunocytochemical and cytogenetic features. Results: When compared to parent BM1 line, BM1R2 exhibited a 17.0-fold resistance to CDDP and cross-resistance to other agents. MRP expression was only observed in BM1R2, whereas MDR1 was expressed in both lines. Notably higher intracellular GST-π and MT levels were observed in BM1R2 cells. MYCN amplifications were 50 and 6 copies in BM1 and BM1R2, respectively, and additional aberrations were observed in chromosome 1 and 2 in BM1R2. Conclusion: It was suggested that GST-π and MT could exert crucial roles on CDDP-resistance in our system. BM1R2 is of great interest for investigating the mechanisms of CDDP-resistance in NB.

Original languageEnglish
Pages (from-to)4049-4057
Number of pages9
JournalAnticancer research
Volume19
Issue number5 B
Publication statusPublished - 1999

Keywords

  • Cisplatin
  • Drug resistance
  • Gultathione-S-transferase
  • Metallothionein
  • Neuroblastoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Establishment and characterization of a cisplatin-resistant human neuroblastoma cell line'. Together they form a unique fingerprint.

Cite this