Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

Hiroyuki Niida; Yuko Katsuno; Misuzu Sengoku; Midori Shimada; Megumi Yukawa; Masae Ikura; Tsuyoshi Ikura; Kazuteru Kohno; Hiroki Shima; Hidekazu Suzuki; Satoshi Tashiro; Makoto Nakanishi

doi:10.1101/gad.1863810

Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

Hiroyuki Niida, Yuko Katsuno, Misuzu Sengoku, Midori Shimada, Megumi Yukawa, Masae Ikura, Tsuyoshi Ikura, Kazuteru Kohno, Hiroki Shima, Hidekazu Suzuki, Satoshi Tashiro, Makoto Nakanishi

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.

Original language	English
Pages (from-to)	333-338
Number of pages	6
Journal	Genes and Development
Volume	24
Issue number	4
DOIs	https://doi.org/10.1101/gad.1863810
Publication status	Published - 2010 Feb 15

Keywords

DNA double-strand breaks
DNA repair
Genomic instability
Ribonucleotide reductase
Tip60
dNTPs

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.1863810

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Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. / Niida, Hiroyuki; Katsuno, Yuko; Sengoku, Misuzu; Shimada, Midori; Yukawa, Megumi; Ikura, Masae; Ikura, Tsuyoshi; Kohno, Kazuteru; Shima, Hiroki; Suzuki, Hidekazu; Tashiro, Satoshi; Nakanishi, Makoto.

In: Genes and Development, Vol. 24, No. 4, 15.02.2010, p. 333-338.

Research output: Contribution to journal › Article › peer-review

Niida, Hiroyuki ; Katsuno, Yuko ; Sengoku, Misuzu ; Shimada, Midori ; Yukawa, Megumi ; Ikura, Masae ; Ikura, Tsuyoshi ; Kohno, Kazuteru ; Shima, Hiroki ; Suzuki, Hidekazu ; Tashiro, Satoshi ; Nakanishi, Makoto. / Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. In: Genes and Development. 2010 ; Vol. 24, No. 4. pp. 333-338.

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