Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

Hiroyuki Niida, Yuko Katsuno, Misuzu Sengoku, Midori Shimada, Megumi Yukawa, Masae Ikura, Tsuyoshi Ikura, Kazuteru Kohno, Hiroki Shima, Hidekazu Suzuki, Satoshi Tashiro, Makoto Nakanishi

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalGenes and Development
Volume24
Issue number4
DOIs
Publication statusPublished - 2010 Feb 15
Externally publishedYes

Keywords

  • DNA double-strand breaks
  • DNA repair
  • Genomic instability
  • Ribonucleotide reductase
  • Tip60
  • dNTPs

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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    Niida, H., Katsuno, Y., Sengoku, M., Shimada, M., Yukawa, M., Ikura, M., Ikura, T., Kohno, K., Shima, H., Suzuki, H., Tashiro, S., & Nakanishi, M. (2010). Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. Genes and Development, 24(4), 333-338. https://doi.org/10.1101/gad.1863810