Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

Hiroyuki Niida; Yuko Katsuno; Misuzu Sengoku; Midori Shimada; Megumi Yukawa; Masae Ikura; Tsuyoshi Ikura; Kazuteru Kohno; Hiroki Shima; Hidekazu Suzuki; Satoshi Tashiro; Makoto Nakanishi

doi:10.1101/gad.1863810

Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

Hiroyuki Niida, Yuko Katsuno, Misuzu Sengoku, Midori Shimada, Megumi Yukawa, Masae Ikura, Tsuyoshi Ikura, Kazuteru Kohno, Hiroki Shima, Hidekazu Suzuki, Satoshi Tashiro, Makoto Nakanishi

Research output: Contribution to journal › Article › peer-review

100 Citations (Scopus)

Abstract

A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.

Original language	English
Pages (from-to)	333-338
Number of pages	6
Journal	Genes and Development
Volume	24
Issue number	4
DOIs	https://doi.org/10.1101/gad.1863810
Publication status	Published - 2010 Feb 15
Externally published	Yes

Keywords

DNA double-strand breaks
DNA repair
Genomic instability
Ribonucleotide reductase
Tip60
dNTPs

ASJC Scopus subject areas

Genetics
Developmental Biology

Access to Document

10.1101/gad.1863810

Cite this

Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. / Niida, Hiroyuki; Katsuno, Yuko; Sengoku, Misuzu; Shimada, Midori; Yukawa, Megumi; Ikura, Masae; Ikura, Tsuyoshi; Kohno, Kazuteru; Shima, Hiroki; Suzuki, Hidekazu; Tashiro, Satoshi; Nakanishi, Makoto.

In: Genes and Development, Vol. 24, No. 4, 15.02.2010, p. 333-338.

Research output: Contribution to journal › Article › peer-review

Niida, Hiroyuki ; Katsuno, Yuko ; Sengoku, Misuzu ; Shimada, Midori ; Yukawa, Megumi ; Ikura, Masae ; Ikura, Tsuyoshi ; Kohno, Kazuteru ; Shima, Hiroki ; Suzuki, Hidekazu ; Tashiro, Satoshi ; Nakanishi, Makoto. / Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. In: Genes and Development. 2010 ; Vol. 24, No. 4. pp. 333-338.

@article{7deb5c1c024e44f985bcbeece8894fb8,

title = "Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase",

abstract = "A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.",

keywords = "DNA double-strand breaks, DNA repair, Genomic instability, Ribonucleotide reductase, Tip60, dNTPs",

author = "Hiroyuki Niida and Yuko Katsuno and Misuzu Sengoku and Midori Shimada and Megumi Yukawa and Masae Ikura and Tsuyoshi Ikura and Kazuteru Kohno and Hiroki Shima and Hidekazu Suzuki and Satoshi Tashiro and Makoto Nakanishi",

year = "2010",

month = feb,

day = "15",

doi = "10.1101/gad.1863810",

language = "English",

volume = "24",

pages = "333--338",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

number = "4",

}

TY - JOUR

T1 - Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

AU - Niida, Hiroyuki

AU - Katsuno, Yuko

AU - Sengoku, Misuzu

AU - Shimada, Midori

AU - Yukawa, Megumi

AU - Ikura, Masae

AU - Ikura, Tsuyoshi

AU - Kohno, Kazuteru

AU - Shima, Hiroki

AU - Suzuki, Hidekazu

AU - Tashiro, Satoshi

AU - Nakanishi, Makoto

PY - 2010/2/15

Y1 - 2010/2/15

N2 - A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.

AB - A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.

KW - DNA double-strand breaks

KW - DNA repair

KW - Genomic instability

KW - Ribonucleotide reductase

KW - Tip60

KW - dNTPs

UR - http://www.scopus.com/inward/record.url?scp=76749114406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76749114406&partnerID=8YFLogxK

U2 - 10.1101/gad.1863810

DO - 10.1101/gad.1863810

M3 - Article

C2 - 20159953

AN - SCOPUS:76749114406

VL - 24

SP - 333

EP - 338

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 4

ER -

Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this