Essential role of Helicobacter pylori urease in gastric colonization: Definite proof using a urease-negative mutant constructed by gene replacement

M. Tsuda, M. Karita, T. Mizote, M. G. Morshed, K. Okita, T. Nakazawa

    Research output: Contribution to journalArticlepeer-review

    40 Citations (Scopus)

    Abstract

    Objective: To investigate the involvement of urease in Helicobacter pylori colonization in the nude mouse stomach by using a genetically defined urease-negative mutant. Methods and results: Through electroporation-mediated gene replacement, one of the urease genes of an H. pylori strain, CYP3401, was disrupted by insertion of a kanamycin-resistance determinant to construct a stable urease-negative mutant, HPT73. Southern analysis confirmed that gene replacement was achieved. The two isogenic strains were introduced into the stomachs of nude mice, and the number of H. pylori and the histological changes in the stomachs were investigated 1 or 4 weeks after the challenge. Gastritis was present in the CPY3401-challenged stomach, from which bacteria indistinguishable from CPY3401 were successfully recovered. In contrast, no gastritis was found in the HPT73-challenged stomach, and H. pylori was not recovered from these stomachs. Conclusion: H. pylori urease is essential for colonization of the nude mouse stomach.

    Original languageEnglish
    Pages (from-to)S49-S52
    JournalEuropean Journal of Gastroenterology and Hepatology, Supplement
    Volume6
    Issue number1
    Publication statusPublished - 1995 Jan 1

    Keywords

    • Colonization
    • Gene replacement mutagenesis
    • Helicobacter pylori
    • Nude mouse model
    • Urease

    ASJC Scopus subject areas

    • Gastroenterology

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