TY - JOUR
T1 - Essential role of Helicobacter pylori urease in gastric colonization
T2 - Definite proof using a urease-negative mutant constructed by gene replacement
AU - Tsuda, M.
AU - Karita, M.
AU - Mizote, T.
AU - Morshed, M. G.
AU - Okita, K.
AU - Nakazawa, T.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Objective: To investigate the involvement of urease in Helicobacter pylori colonization in the nude mouse stomach by using a genetically defined urease-negative mutant. Methods and results: Through electroporation-mediated gene replacement, one of the urease genes of an H. pylori strain, CYP3401, was disrupted by insertion of a kanamycin-resistance determinant to construct a stable urease-negative mutant, HPT73. Southern analysis confirmed that gene replacement was achieved. The two isogenic strains were introduced into the stomachs of nude mice, and the number of H. pylori and the histological changes in the stomachs were investigated 1 or 4 weeks after the challenge. Gastritis was present in the CPY3401-challenged stomach, from which bacteria indistinguishable from CPY3401 were successfully recovered. In contrast, no gastritis was found in the HPT73-challenged stomach, and H. pylori was not recovered from these stomachs. Conclusion: H. pylori urease is essential for colonization of the nude mouse stomach.
AB - Objective: To investigate the involvement of urease in Helicobacter pylori colonization in the nude mouse stomach by using a genetically defined urease-negative mutant. Methods and results: Through electroporation-mediated gene replacement, one of the urease genes of an H. pylori strain, CYP3401, was disrupted by insertion of a kanamycin-resistance determinant to construct a stable urease-negative mutant, HPT73. Southern analysis confirmed that gene replacement was achieved. The two isogenic strains were introduced into the stomachs of nude mice, and the number of H. pylori and the histological changes in the stomachs were investigated 1 or 4 weeks after the challenge. Gastritis was present in the CPY3401-challenged stomach, from which bacteria indistinguishable from CPY3401 were successfully recovered. In contrast, no gastritis was found in the HPT73-challenged stomach, and H. pylori was not recovered from these stomachs. Conclusion: H. pylori urease is essential for colonization of the nude mouse stomach.
KW - Colonization
KW - Gene replacement mutagenesis
KW - Helicobacter pylori
KW - Nude mouse model
KW - Urease
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M3 - Article
C2 - 7735935
AN - SCOPUS:0028925321
VL - 6
SP - S49-S52
JO - European Journal of Gastroenterology and Hepatology, Supplement
JF - European Journal of Gastroenterology and Hepatology, Supplement
SN - 0969-9163
IS - 1
ER -