Escherichia coli yjjPB genes encode a succinate transporter important for succinate production

Keita Fukui, Kei Nanatani, Yoshihiko Hara, Suguru Yamakami, Daiki Yahagi, Akito Chinen, Mitsunori Tokura, Keietsu Abe

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Under anaerobic conditions, Escherichia coli produces succinate from glucose via the reductive tricarboxylic acid cycle. To date, however, no genes encoding succinate exporters have been established in E. coli. Therefore, we attempted to identify genes encoding succinate exporters by screening an E. coli MG1655 genome library. We identified the yjjPB genes as candidates encoding a succinate transporter, which enhanced succinate production in Pantoea ananatis under aerobic conditions. A complementation assay conducted in Corynebacterium glutamicum strain AJ110655ΔsucE1 demonstrated that both YjjP and YjjB are required for the restoration of succinate production. Furthermore, deletion of yjjPB decreased succinate production in E. coli by 70% under anaerobic conditions. Taken together, these results suggest that YjjPB constitutes a succinate transporter in E. coli and that the products of both genes are required for succinate export.

Original languageEnglish
Pages (from-to)1837-1844
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume81
Issue number9
DOIs
Publication statusPublished - 2017

Keywords

  • Aerobic conditions
  • Anaerobic conditions
  • Escherichia coli
  • Succinate production
  • Succinate transporter

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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