ERdj3B-mediated quality control maintains anther development at high temperatures

Masaya Yamamoto, Shuhei Uji, Tomoyuki Sugiyama, Tomoaki Sakamoto, Seisuke Kimura, Toshiya Endo, Shuh Ichi Nishikawa

Research output: Contribution to journalArticle

Abstract

Pollen development is highly sensitive to heat stress, which impairs cellular proteostasis by causing misfolded proteins to accumulate. Therefore, each cellular compartment possesses a dedicated protein quality control system. An elaborate quality control system involving molecular chaperones, including immunoglobulin-binding protein (BiP), heat shock protein70, and regulatory J domain-containing cochaperones (J proteins), allows the endoplasmic reticulum (ER) to withstand a large influx of proteins. Here, we found that Arabidopsis (Arabidopsis thaliana) mutants of ER-localized DnaJ family 3B (ERdj3B), one of three ER-resident J proteins involved in ER quality control, produced few seeds at high temperatures (29°C) due to defects in anther development. This temperature-sensitive fertility defect is specific to the defective interactions of BiP with ERdj3B but not with the other two J proteins, indicating functional differences between ERdj3B and the other J proteins. RNA sequencing analysis revealed that heat stress affects pollen development in both wild-type and mutant buds, but the erdj3b mutant is more susceptible, possibly due to defects in ER quality control. Our results highlight the importance of a specific ER quality control factor, ERdj3B, for plant reproduction, particularly anther development, at high temperatures.

Original languageEnglish
Pages (from-to)1979-1990
Number of pages12
JournalPlant physiology
Volume182
Issue number4
DOIs
Publication statusPublished - 2020 Apr

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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    Yamamoto, M., Uji, S., Sugiyama, T., Sakamoto, T., Kimura, S., Endo, T., & Nishikawa, S. I. (2020). ERdj3B-mediated quality control maintains anther development at high temperatures. Plant physiology, 182(4), 1979-1990. https://doi.org/10.1104/PP.19.01356