EPR imaging of reducing activity in Nrf2 transcriptional factor-deficient mice

Aki Hirayama, Keigyou Yoh, Sohji Nagase, Atsushi Ueda, Ken Itoh, Naoki Morito, Kouichi Hirayama, Satoru Takahashi, Masayuki Yamamoto, Akio Koyama

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Mice that lack the Nrf2 (NF-E2-related factor 2) transcription factor develop a lupus-like autoimmune nephritis. The tissue-reducing activity of Nrf2-deficient mice was evaluated using a combination of real-time EPR imaging and spin probe kinetic analysis. Substantial delay in the spin probe 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (Carbamoyl-PROXYL) disappearance in the liver and kidneys of Nrf2-deficient mice was observed by EPR imaging. The half-life of the spin probe in the upper abdominal area was prolonged in both the Nrf2-deficient mice and in aged mice. The combination of Nrf2 deficiency and aging in female mice resulted in the most prolonged half-life of disappearance, which was four times longer than that of juvenile female mice with a wild-type genotype. These results indicate that the low reducing activity in these organs is brought about by both Nrf2 deficiency and the aging process, and it may play a key role in the onset of autoimmune nephritis. This combination of the EPR imaging and half-life analysis appears to be a very powerful tool in the real-time analysis of reducing activity.

Original languageEnglish
Pages (from-to)1236-1242
Number of pages7
JournalFree Radical Biology and Medicine
Volume34
Issue number10
DOIs
Publication statusPublished - 2003 May 15
Externally publishedYes

Keywords

  • Carbamoyl-PROXYL
  • EPR
  • EPR imaging
  • Free radicals
  • Lupus nephritis
  • Nrf2

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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