Epizootology and experimental infection of Yokose virus in bats

Shumpei Watanabe, Tsutomu Omatsu, Mary E.G. Miranda, Joseph S. Masangkay, Naoya Ueda, Maiko Endo, Kentaro Kato, Yukinobu Tohya, Yasuhiro Yoshikawa, Hiroomi Akashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.

Original languageEnglish
Pages (from-to)25-36
Number of pages12
JournalComparative Immunology, Microbiology and Infectious Diseases
Issue number1
Publication statusPublished - 2010 Jan
Externally publishedYes


  • Anti-bat IgG
  • Bat
  • Chiroptera
  • Experimental infection
  • Yokose virus

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases


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