Epizootology and experimental infection of Yokose virus in bats

Shumpei Watanabe; Tsutomu Omatsu; Mary E.G. Miranda; Joseph S. Masangkay; Naoya Ueda; Maiko Endo; Kentaro Kato; Yukinobu Tohya; Yasuhiro Yoshikawa; Hiroomi Akashi

doi:10.1016/j.cimid.2008.07.008

Epizootology and experimental infection of Yokose virus in bats

Shumpei Watanabe, Tsutomu Omatsu, Mary E.G. Miranda, Joseph S. Masangkay, Naoya Ueda, Maiko Endo, Kentaro Kato, Yukinobu Tohya, Yasuhiro Yoshikawa, Hiroomi Akashi

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.

Original language	English
Pages (from-to)	25-36
Number of pages	12
Journal	Comparative Immunology, Microbiology and Infectious Diseases
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.cimid.2008.07.008
Publication status	Published - 2010 Jan
Externally published	Yes

Keywords

Anti-bat IgG
Bat
Chiroptera
ELISA
Experimental infection
Yokose virus

ASJC Scopus subject areas

Microbiology
Immunology and Allergy
Immunology
veterinary(all)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cimid.2008.07.008

Cite this

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title = "Epizootology and experimental infection of Yokose virus in bats",

abstract = "To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.",

keywords = "Anti-bat IgG, Bat, Chiroptera, ELISA, Experimental infection, Yokose virus",

author = "Shumpei Watanabe and Tsutomu Omatsu and Miranda, {Mary E.G.} and Masangkay, {Joseph S.} and Naoya Ueda and Maiko Endo and Kentaro Kato and Yukinobu Tohya and Yasuhiro Yoshikawa and Hiroomi Akashi",

note = "Funding Information: We thank Dr. Tomohiko Takasaki of the National Institute of Infectious Diseases of Japan for providing the Oita-36 strain of YOKV, and the members of the Veterinary Research Department of the Research Institute for Tropical Medicine in the Philippines for their assistance in collecting and processing specimens. This study was supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan.",

year = "2010",

month = jan,

doi = "10.1016/j.cimid.2008.07.008",

language = "English",

volume = "33",

pages = "25--36",

journal = "Comparative Immunology, Microbiology and Infectious Diseases",

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AU - Watanabe, Shumpei

AU - Omatsu, Tsutomu

AU - Miranda, Mary E.G.

AU - Masangkay, Joseph S.

AU - Ueda, Naoya

AU - Endo, Maiko

AU - Kato, Kentaro

AU - Tohya, Yukinobu

AU - Yoshikawa, Yasuhiro

AU - Akashi, Hiroomi

N1 - Funding Information: We thank Dr. Tomohiko Takasaki of the National Institute of Infectious Diseases of Japan for providing the Oita-36 strain of YOKV, and the members of the Veterinary Research Department of the Research Institute for Tropical Medicine in the Philippines for their assistance in collecting and processing specimens. This study was supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2010/1

Y1 - 2010/1

N2 - To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.

AB - To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.

KW - Anti-bat IgG

KW - Bat

KW - Chiroptera

KW - ELISA

KW - Experimental infection

KW - Yokose virus

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Epizootology and experimental infection of Yokose virus in bats

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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