Epitope Mapping of Monoclonal Antibody PMab-48 Against Dog Podoplanin

Shinji Yamada, Mika K. Kaneko, Shunsuke Itai, Yao Wen Chang, Takuro Nakamura, Miyuki Yanaka, Satoshi Ogasawara, Takeshi Murata, Hiroaki Uchida, Hideaki Tahara, Hiroyuki Harada, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on normal renal podocytes, pulmonary type I alveolar cells, and lymphatic endothelial cells. Increased expression of PDPN in cancers is associated with poor prognosis and hematogenous metastasis through interactions with C-type lectin-like receptor 2 (CLEC-2) on platelets. We previously reported a novel PMab-48 antibody, which is an anti-dog PDPN (dPDPN) monoclonal antibody (mAb) recognizing PDPN expressed in lymphatic endothelial cells. However, the binding epitope of PMab-48 is yet to be clarified. In this study, an enzyme-linked immunosorbent assay and flow cytometry were used to investigate epitopes of PMab-48. The results revealed that the critical epitope of PMab-48 comprises Asp29, Asp30, Ile31, Ile32, and Pro33 of dPDPN.

Original languageEnglish
Pages (from-to)162-165
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume37
Issue number3
DOIs
Publication statusPublished - 2018 Jun

Keywords

  • dPDPN
  • dog podoplanin
  • epitope mapping
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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