Epitope Mapping of Monoclonal Antibody PMab-38 Against Dog Podoplanin

Yao Wen Chang, Shinji Yamada, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is extensively expressed by normal lymphatic endothelial cells, renal podocytes, and pulmonary type I alveolar cells. Nevertheless, increased expression of PDPN in malignant tumors not only associates with poor prognosis but also facilitates hematogenous metastasis through interaction with C-type lectin-like receptor-2 presented on platelets, followed by PDPN-mediated platelet activation. We previously reported a novel PMab-38 antibody, an anti-dog PDPN (dPDPN) monoclonal antibody, which specifically recognizes PDPN in squamous cell carcinomas melanomas and cancer-associated fibroblasts in canine cancer tissues. However, the specific binding with the epitope of PMab-38 remains undefined. In this study, flow cytometry was utilized to investigate the epitope of PMab-38, which was determined using a series of deletion or point mutants of dPDPN. The results revealed that the critical epitope of PMab-38 is Tyr67 and Glu68 of dPDPN.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume36
Issue number6
DOIs
Publication statusPublished - 2017 Dec

Keywords

  • dog PDPN
  • epitope
  • podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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