Epitope Mapping of Antipig Podoplanin Monoclonal Antibody PMab-213

Shinji Yamada, Shunsuke Itai, Yoshikazu Furusawa, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on lymphatic endothelial cells, podocytes of the kidneys, and type I alveolar cells of the lungs. PDPN, a platelet aggregation-inducing factor, comprises three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) in the N-terminus and PLAG-like domains in the middle of the PDPN protein. We have previously reported a mouse antipig PDPN (pPDPN) monoclonal antibody (mAb) clone, PMab-213, which was developed using the Cell-Based Immunization and Screening (CBIS) method. PMab-213 is very useful in flow cytometry, Western blotting, and immunohistochemical analyses; however, the binding epitope of PMab-213, which was developed by CBIS method, remains to be elucidated. Therefore, this study aimed to investigate the epitope of PMab-213 using enzyme-linked immunosorbent assay, flow cytometry, and immunohistochemical analyses. The results revealed that the critical epitopes of PMab-213 are Pro53, Arg54, Arg56, and Tyr60 of pPDPN.

Original languageEnglish
Pages (from-to)224-229
Number of pages6
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number5
DOIs
Publication statusPublished - 2019 Oct

Keywords

  • PDPN
  • PMab-213
  • epitope mapping
  • podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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