Epitope Mapping of Anti-Tiger Podoplanin Monoclonal Antibody PMab-231

Junko Takei, Shunsuke Itai, Yoshikazu Furusawa, Shinji Yamada, Takuro Nakamura, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Podoplanin (PDPN) is expressed on podocytes of the kidneys, type I alveolar cells of the lungs, and lymphatic endothelial cells. PDPN comprises three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) in the N-terminus and PLAG-like domains in the middle of the PDPN protein. We have previously reported on an anti-tiger PDPN (tigPDPN) monoclonal antibody (mAb), PMab-231, which was developed using the Cell-Based Immunization and Screening (CBIS) method. PMab-231 is very useful in flow cytometry, Western blotting, and immunohistochemical analyses; however, the binding epitope of PMab-231 remains to be elucidated. This study aimed to investigate the epitopes of PMab-231, which was developed by CBIS method, using enzyme-linked immunosorbent assay. The results revealed that the critical epitopes of PMab-231 are Glu29, Asp30, Asp31, Ile32, Met33, Thr34, Pro35, Gly36, and Glu38 of tigPDPN, which is corresponding to PLAG1/2. The findings of our study can be applied to the production of more functional anti-tigPDPN mAbs.

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number3
DOIs
Publication statusPublished - 2019 Jun

Keywords

  • PDPN
  • PMab-231
  • epitope mapping
  • podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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