Epitope mapping of anti-telomerase reverse transcriptase monoclonal antibodies

Yoshikazu Furusawa, Shunsuke Itai, Shinji Yamada, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticle

Abstract

Telomerase reverse transcriptase (TERT) is the main catalytic subunit of telomerase-associated protein machinery. Upregulation of TERT at the transcriptional level results in immortal cell phenotype associated with cancer. To date we have developed eight anti-TERT monoclonal antibodies (mAbs) (TMab-4, TMab-5, TMab-6, TMab-7, TMab-9, TMab-10, TMab-11, and TMab-12) by immunizing mice with synthetic peptides (302-QHHAGPPSTSRPPRPWDTPC-321) of TERT. We further characterized those epitopes using enzyme-linked immunosorbent assay, and here we discuss the critical epitope of an anti-TERT mAb, which is applicable for immunohistochemical analysis.

Original languageEnglish
Pages (from-to)185-187
Number of pages3
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume37
Issue number4
DOIs
Publication statusPublished - 2018 Aug

Keywords

  • TERT
  • epitope mapping
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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