Epitope analysis of the cerebrospinal fluid IgG in HTLV-I associated myelopathy patients using phage display method

J. Fujimori, I. Nakashima, K. Fujihara, J. Feng, M. Yamamoto, N. Yamamoto, N. Begum, Y. Sagara, H. Shiraki, Y. Shiga, J. Onodera, S. Sato, S. Takase, M. Asano, M. Endo, Y. Itoyama

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We, for the first time, analyzed the binding motifs of immunoglobulin G (IgG) in the cerebrospinal fluid (CSF) of human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients with a phage library displaying 12-mer random peptides. As a result, the sequences highly homologous to HTLV-I gp46 192-199, 237-243 and 255-261 were the common linear epitopes of high affinity- IgG exclusively detected in both CSF and sera of the patients. These IgG responses were confirmed with corresponding HTLV-I peptides and serum antibody titers significantly correlated with disease severity or duration. Gp46 237-243 has not been detected in previous enzyme-linked immunosorbent assay (ELISA) studies using bound longer peptides, suggesting the usefulness of the phage display method.

Original languageEnglish
Pages (from-to)140-146
Number of pages7
JournalJournal of Neuroimmunology
Volume152
Issue number1-2
DOIs
Publication statusPublished - 2004 Jul

Keywords

  • Human T lymphotropic virus type I
  • Immunoglobulin G
  • Phage display

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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