TY - JOUR
T1 - Epithelial modulation of leukotriene-C4-induced human tracheal smooth muscle contraction
AU - Yamaya, Mutsuo
AU - Sekizawa, Kiyohisa
AU - Yamauchi, Kohei
AU - Hoshi, Hiroki
AU - Sawai, Takashi
AU - Sasaki, Hidetada
PY - 1995/3
Y1 - 1995/3
N2 - We investigated the role of epithelium in smooth muscle contraction induced by leukotriene C4 (LTC4) in isolated human trachea. The contractile response to LTC4 was potentiated by a gamma-glutamyl transpeptidase (gamma- GTP) inhibitor L-serine borate and by removal of the epithelium. Both L- serine borate (4.5 x 10-2 M) and removal of the epithelium shifted the concentration-response curves to LTC4 to lower concentrations by 1.2 and 1.0 log units, respectively. Incubation of cultured human tracheal epithelial cells with either LTC4 or LTD4 resulted in the formation of LTD4, or LTE4. The isolated epithelial cells and cultured epithelial cells contained gamma-GTP activity. Histochemical study indicated that gamma-GTP-like activity was present in the epithelial, submucosal, and smooth muscle layers. These findings suggest that the epithelium modulates the contractile response to LTC4 in human trachea by LT-degrading enzymes.
AB - We investigated the role of epithelium in smooth muscle contraction induced by leukotriene C4 (LTC4) in isolated human trachea. The contractile response to LTC4 was potentiated by a gamma-glutamyl transpeptidase (gamma- GTP) inhibitor L-serine borate and by removal of the epithelium. Both L- serine borate (4.5 x 10-2 M) and removal of the epithelium shifted the concentration-response curves to LTC4 to lower concentrations by 1.2 and 1.0 log units, respectively. Incubation of cultured human tracheal epithelial cells with either LTC4 or LTD4 resulted in the formation of LTD4, or LTE4. The isolated epithelial cells and cultured epithelial cells contained gamma-GTP activity. Histochemical study indicated that gamma-GTP-like activity was present in the epithelial, submucosal, and smooth muscle layers. These findings suggest that the epithelium modulates the contractile response to LTC4 in human trachea by LT-degrading enzymes.
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U2 - 10.1164/ajrccm.151.3.7881688
DO - 10.1164/ajrccm.151.3.7881688
M3 - Article
C2 - 7881688
AN - SCOPUS:0028913455
VL - 151
SP - 892
EP - 894
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 3 I
ER -