Epithelial induction of serum amyloid A in experimental mucosal inflammation

Kouhei Fukushima, Hitoshi Ogawa, Taku Kitayama, Toshiyuki Yamada, Hiroo Naito, Yuji Funayama, Seiki Matsuno, Iwao Sasaki

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We previously demonstrated epithelial induction of serum amyloid A in germ-free mice inoculated with luminal bacteria. The aims of the present study were to investigate the role of luminal bacteria and mucosal inflammation in epithelial expression of this acute-phase protein using germ-free and dextran sulfate sodium-treated mice in viva and HT29 cells in vitro. Immunoreactivity for serum amyloid A was detected in the epithelium of esophagus, stomach, duodenum and rectum regardless of the presence or absence of luminal bacteria. Administration of dextran sulfate sodium resulted in colonic epithelial induction of serum amyloid A at the mRNA and protein levels in parallel with the progression of mucosal inflammation. Epithelial induction of serum amyloid A is possibly relevant to mucosal inflammation because that was observed in bacteria-reconstituted and dextran sulfate sodium-induced colitis in vivo and because interleukin-1β and lipopolysaccharide induced its mRNA in vitro.

Original languageEnglish
Pages (from-to)1438-1446
Number of pages9
JournalDigestive Diseases and Sciences
Volume47
Issue number7
DOIs
Publication statusPublished - 2002

Keywords

  • Dextran sulfate sodium
  • Epithelial cell
  • Mucosal inflammation
  • Serum amyloid A

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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