Epigallocatechin gallate decreases the micellar solubility of cholesterol via specific interaction with phosphatidylcholine

Makoto Kobayashi, Masato Nishizawa, Nao Inoue, Takahiro Hosoya, Masahito Yoshida, Yuichi Ukawa, Yuko M. Sagesaka, Takayuki Doi, Tsutomu Nakayama, Shigenori Kumazawa, Ikuo Ikeda

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

The mechanisms underlying the effect of epigallocatechin gallate (EGCG) on the micellar solubility of cholesterol were examined. EGCG eliminated both cholesterol and phosphatidylcholine (PC) from bile salt micelles in a dose-dependent manner in vitro. When the bile salt micelles contained a phospholipid other than PC, neither cholesterol nor the phospholipid was eliminated following the addition of EGCG. When vesicles comprised of various phospholipids were prepared and, EGCG was added to the vesicles, EGCG effectively and exclusively eliminated only PC. An intermolecular nuclear Overhauser effect (NOE) was observed between PC and EGCG in bile salt micelles with EGCG added, but not between cholesterol and EGCG, by using a NOE-correlated spectroscopy nuclear magnetic resonance method. The results of binding analyses using surface plasmon resonance (SPR) showed that EGCG did not bind to cholesterol. These observations strongly suggest that EGCG decreases the micellar solubility of cholesterol via specific interaction with PC.

Original languageEnglish
Pages (from-to)2881-2890
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number13
DOIs
Publication statusPublished - 2014 Apr 2

Keywords

  • epigallocatechin gallate
  • micellar solubility of cholesterol
  • nuclear Overhauser effect
  • phosphatidylcholine
  • surface plasmon resonance

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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