Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR

Longzheng Yu, Shoufa Zhang, Wanfeng Liang, Chunmei Jin, Lijun Jia, Yuzi Luo, Yan Li, Shinuo Cao, Junya Yamagishi, Yoshifumi Nishikawa, Suguru Kawano, Kozo Fujisaki, Xuenan Xuan

Research output: Contribution to journalComment/debatepeer-review

10 Citations (Scopus)

Abstract

An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.

Original languageEnglish
Pages (from-to)1509-1512
Number of pages4
JournalJournal of Veterinary Medical Science
Volume73
Issue number11
DOIs
Publication statusPublished - 2011 Nov
Externally publishedYes

Keywords

  • Major piroplasm surface protein
  • PCR
  • Theileria

ASJC Scopus subject areas

  • veterinary(all)

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