TY - JOUR
T1 - Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR
AU - Yu, Longzheng
AU - Zhang, Shoufa
AU - Liang, Wanfeng
AU - Jin, Chunmei
AU - Jia, Lijun
AU - Luo, Yuzi
AU - Li, Yan
AU - Cao, Shinuo
AU - Yamagishi, Junya
AU - Nishikawa, Yoshifumi
AU - Kawano, Suguru
AU - Fujisaki, Kozo
AU - Xuan, Xuenan
PY - 2011/11
Y1 - 2011/11
N2 - An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.
AB - An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.
KW - Major piroplasm surface protein
KW - PCR
KW - Theileria
UR - http://www.scopus.com/inward/record.url?scp=82955219670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82955219670&partnerID=8YFLogxK
U2 - 10.1292/jvms.10-0507
DO - 10.1292/jvms.10-0507
M3 - Comment/debate
C2 - 21737967
AN - SCOPUS:82955219670
VL - 73
SP - 1509
EP - 1512
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
SN - 0916-7250
IS - 11
ER -