Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR

Longzheng Yu; Shoufa Zhang; Wanfeng Liang; Chunmei Jin; Lijun Jia; Yuzi Luo; Yan Li; Shinuo Cao; Junya Yamagishi; Yoshifumi Nishikawa; Suguru Kawano; Kozo Fujisaki; Xuenan Xuan

doi:10.1292/jvms.10-0507

Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR

Longzheng Yu, Shoufa Zhang, Wanfeng Liang, Chunmei Jin, Lijun Jia, Yuzi Luo, Yan Li, Shinuo Cao, Junya Yamagishi, Yoshifumi Nishikawa, Suguru Kawano, Kozo Fujisaki, Xuenan Xuan

Research output: Contribution to journal › Comment/debate › peer-review

11 Citations (Scopus)

Abstract

An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.

Original language	English
Pages (from-to)	1509-1512
Number of pages	4
Journal	Journal of Veterinary Medical Science
Volume	73
Issue number	11
DOIs	https://doi.org/10.1292/jvms.10-0507
Publication status	Published - 2011 Nov
Externally published	Yes

Keywords

Major piroplasm surface protein
PCR
Theileria

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.10-0507

Cite this

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title = "Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR",

abstract = "An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.",

keywords = "Major piroplasm surface protein, PCR, Theileria",

author = "Longzheng Yu and Shoufa Zhang and Wanfeng Liang and Chunmei Jin and Lijun Jia and Yuzi Luo and Yan Li and Shinuo Cao and Junya Yamagishi and Yoshifumi Nishikawa and Suguru Kawano and Kozo Fujisaki and Xuenan Xuan",

year = "2011",

month = nov,

doi = "10.1292/jvms.10-0507",

language = "English",

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pages = "1509--1512",

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TY - JOUR

T1 - Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR

AU - Yu, Longzheng

AU - Zhang, Shoufa

AU - Liang, Wanfeng

AU - Jin, Chunmei

AU - Jia, Lijun

AU - Luo, Yuzi

AU - Li, Yan

AU - Cao, Shinuo

AU - Yamagishi, Junya

AU - Nishikawa, Yoshifumi

AU - Kawano, Suguru

AU - Fujisaki, Kozo

AU - Xuan, Xuenan

PY - 2011/11

Y1 - 2011/11

N2 - An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.

AB - An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.

KW - Major piroplasm surface protein

KW - PCR

KW - Theileria

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U2 - 10.1292/jvms.10-0507

DO - 10.1292/jvms.10-0507

M3 - Comment/debate

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AN - SCOPUS:82955219670

VL - 73

SP - 1509

EP - 1512

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 11

ER -

Epidemiological survey of theileria parasite infection of cattle in northeast china by allele-specific PCR

Abstract

Keywords

ASJC Scopus subject areas

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