TY - JOUR
T1 - Epidemiological analysis of nosocomial MRSA outbreaks using phage open-reading frame typing in a tertiary-care hospital
AU - Kawamura, Hideki
AU - Tokuda, Koichi
AU - Imuta, Naoko
AU - Kubota, Tomohiro
AU - Koriyama, Toyoyasu
AU - Miyanohara, Hiroaki
AU - Hashiguchi, Teruto
AU - Kawano, Yoshifumi
AU - Nishi, Junichiro
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI,grant number 25870567.
Publisher Copyright:
© 2016,National Institute of Health. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Pulsed-field gel electrophoresis (PFGE) is a reliable method for analyzing outbreaks of methicillin-resistant Staphylococcus aureus (MRSA); however,it is time-consuming and technically demanding. A new strain-differentiation method for MRSA,namely phage open reading frame (ORF) typing (POT),is a rapid PCR-based technique,in which the ORFs of lysogenized phage genomes in MRSA are amplified. The aim of this study was to evaluate the utility of the POT method for epidemiological analysis of nosocomial MRSA outbreaks. Forty-four strains from 12 episodes of 3 or more nosocomial MRSA infections in 1 ward within a 4-week period were characterized using PFGE and POT methods. The strains were classified into 16 distinct types using POT and 19 subtypes using PFGE. We defined an outbreak as 3 or more new MRSA infections caused by strains with indistinguishable genetic patterns. The identification of 11 (91.7z) episodes by PFGE,including 4 outbreaks and 7 sporadic events,was consistent with the results of POT analysis. These results suggest that POT is a useful epidemiological tool for evaluating nosocomial MRSA outbreaks.
AB - Pulsed-field gel electrophoresis (PFGE) is a reliable method for analyzing outbreaks of methicillin-resistant Staphylococcus aureus (MRSA); however,it is time-consuming and technically demanding. A new strain-differentiation method for MRSA,namely phage open reading frame (ORF) typing (POT),is a rapid PCR-based technique,in which the ORFs of lysogenized phage genomes in MRSA are amplified. The aim of this study was to evaluate the utility of the POT method for epidemiological analysis of nosocomial MRSA outbreaks. Forty-four strains from 12 episodes of 3 or more nosocomial MRSA infections in 1 ward within a 4-week period were characterized using PFGE and POT methods. The strains were classified into 16 distinct types using POT and 19 subtypes using PFGE. We defined an outbreak as 3 or more new MRSA infections caused by strains with indistinguishable genetic patterns. The identification of 11 (91.7z) episodes by PFGE,including 4 outbreaks and 7 sporadic events,was consistent with the results of POT analysis. These results suggest that POT is a useful epidemiological tool for evaluating nosocomial MRSA outbreaks.
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U2 - 10.7883/yoken.JJID.2015.320
DO - 10.7883/yoken.JJID.2015.320
M3 - Article
C2 - 26743145
AN - SCOPUS:84996564362
VL - 69
SP - 523
EP - 524
JO - Japanese medical journal
JF - Japanese medical journal
SN - 1344-6304
IS - 6
ER -
