Ephedorae herba decreases lipopolysaccharide-induced cyclooxgenase-2 protein expression and NF-κB-dependent transcription in C6 rat glioma cells

Kohichi Aoki, Tohru Yamakuni, Masaaki Yoshida, Yasushi Ohizumi

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We studied the effects of Mao (Ephedrae herba) on lipopolysaccharide (LPS)-induced expression of inducible cyclooxygenase (COX-2) in C6 rat glioma cells. Western blot analysis showed that Mao inhibited LPS-induced expression of COX-2 protein, but not that of constitutive cyclooxygenase. Reporter gene assays showed that Mao reduced LPS-inducible NF-κB-dependent transcription that plays a crucial role in induction of COX-2 gene expression. This crude drug decreased LPS-induced IκBα degradation in a concentration-dependent manner without affecting LPS-induced IκB phosphorylation. These results suggest that Mao prevents LPS-induced NF-κB-dependent transcription by inhibiting IκB degradation and suppresses an increase in COX-2 protein expression in C6 cells.

Original languageEnglish
Pages (from-to)327-330
Number of pages4
JournalJournal of Pharmacological Sciences
Volume98
Issue number3
DOIs
Publication statusPublished - 2005

Keywords

  • COX-2 gene expression
  • Ephedrae herba
  • NF-κB-dependent transcription

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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