Eosinophil adhesion to cultured airway Epithelial cells from Guinea pig lungs

T. Masuda, S. Shimura, N. Maruyama, M. Nara, K. Shirato

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1 Citation (Scopus)

Abstract

We examined the effects of histamine, tumor necrosis factor-α (TNFα), interferon-γ (IFNγ), indomethacin and dexamethasone on the adherence of eosmophils to airway epithelial cells. Epithelial cells were isolated from guinea pig trachea through enzymatic digestion, and cultured until they showed confluence. Eosinophils were taken by peritoneal lavage from guinea pigs sensitized with horse serum and purified. The adhesion of eosinophils to cultured epithelial cells was examined using51Cr. The percentage adhesion was obtained by dividing counts per minute of lysed eosinophils adhered to epithelial cells by suspended eosinophils. Histamine significantly increased the adhesion of eosinophils to epithelial cells, while TNFα or IFNγ did not significantly alter the adhesion. Indomethacin significantly inhibited the adhesion of eosinophils to epithelial cells in both nontreated and histamine-induced conditions. Dexamethasone also reduced adhesion, although it did not reach statistical significance. These findings indicate that histamine induced eosinophil adherence to airway epithelial cells, suggesting a role of endogenous prostaglandins in adherence.

Original languageEnglish
Pages (from-to)358-362
Number of pages5
JournalRespiration
Volume63
Issue number6
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • Adhesion
  • Airway epithelial cell
  • Eosinophil
  • Guinea pig

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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