TY - JOUR
T1 - Eosinophil adhesion to cultured airway Epithelial cells from Guinea pig lungs
AU - Masuda, T.
AU - Shimura, S.
AU - Maruyama, N.
AU - Nara, M.
AU - Shirato, K.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - We examined the effects of histamine, tumor necrosis factor-α (TNFα), interferon-γ (IFNγ), indomethacin and dexamethasone on the adherence of eosmophils to airway epithelial cells. Epithelial cells were isolated from guinea pig trachea through enzymatic digestion, and cultured until they showed confluence. Eosinophils were taken by peritoneal lavage from guinea pigs sensitized with horse serum and purified. The adhesion of eosinophils to cultured epithelial cells was examined using51Cr. The percentage adhesion was obtained by dividing counts per minute of lysed eosinophils adhered to epithelial cells by suspended eosinophils. Histamine significantly increased the adhesion of eosinophils to epithelial cells, while TNFα or IFNγ did not significantly alter the adhesion. Indomethacin significantly inhibited the adhesion of eosinophils to epithelial cells in both nontreated and histamine-induced conditions. Dexamethasone also reduced adhesion, although it did not reach statistical significance. These findings indicate that histamine induced eosinophil adherence to airway epithelial cells, suggesting a role of endogenous prostaglandins in adherence.
AB - We examined the effects of histamine, tumor necrosis factor-α (TNFα), interferon-γ (IFNγ), indomethacin and dexamethasone on the adherence of eosmophils to airway epithelial cells. Epithelial cells were isolated from guinea pig trachea through enzymatic digestion, and cultured until they showed confluence. Eosinophils were taken by peritoneal lavage from guinea pigs sensitized with horse serum and purified. The adhesion of eosinophils to cultured epithelial cells was examined using51Cr. The percentage adhesion was obtained by dividing counts per minute of lysed eosinophils adhered to epithelial cells by suspended eosinophils. Histamine significantly increased the adhesion of eosinophils to epithelial cells, while TNFα or IFNγ did not significantly alter the adhesion. Indomethacin significantly inhibited the adhesion of eosinophils to epithelial cells in both nontreated and histamine-induced conditions. Dexamethasone also reduced adhesion, although it did not reach statistical significance. These findings indicate that histamine induced eosinophil adherence to airway epithelial cells, suggesting a role of endogenous prostaglandins in adherence.
KW - Adhesion
KW - Airway epithelial cell
KW - Eosinophil
KW - Guinea pig
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U2 - 10.1159/000196577
DO - 10.1159/000196577
M3 - Article
C2 - 8933654
AN - SCOPUS:0029858507
VL - 63
SP - 358
EP - 362
JO - Schweizerische Zeitschrift für Tuberkulose und Pneumonologie. Revue suisse de la tuberculose et de pneumonologie. Rivista svizzera della tubercolosi e della pneumonologia
JF - Schweizerische Zeitschrift für Tuberkulose und Pneumonologie. Revue suisse de la tuberculose et de pneumonologie. Rivista svizzera della tubercolosi e della pneumonologia
SN - 0025-7931
IS - 6
ER -