Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction

Jun Ichi Anzai, Yuka Kobayashi, Yasuhiro Suzuki, Hiroki Takeshita, Qiang Chen, Tetsuo Osa, Tomonori Hoshi, Xiao Yan Du

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed.

Original languageEnglish
Pages (from-to)3-9
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume52
Issue number1-2
DOIs
Publication statusPublished - 1998 Sep 15

Keywords

  • Avidin-biotin system
  • Biotin-labeled enzyme
  • Enzyme multilayer
  • Enzyme sensor
  • Glucose sensor
  • Lactate sensor

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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