Abstract
An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed.
Original language | English |
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Pages (from-to) | 3-9 |
Number of pages | 7 |
Journal | Sensors and Actuators, B: Chemical |
Volume | 52 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 1998 Sep 15 |
Externally published | Yes |
Keywords
- Avidin-biotin system
- Biotin-labeled enzyme
- Enzyme multilayer
- Enzyme sensor
- Glucose sensor
- Lactate sensor
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry