Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction

Jun Ichi Anzai; Yuka Kobayashi; Yasuhiro Suzuki; Hiroki Takeshita; Qiang Chen; Tetsuo Osa; Tomonori Hoshi; Xiao Yan Du

doi:10.1016/S0925-4005(98)00248-2

Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction

Jun Ichi Anzai, Yuka Kobayashi, Yasuhiro Suzuki, Hiroki Takeshita, Qiang Chen, Tetsuo Osa, Tomonori Hoshi, Xiao Yan Du

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed.

Original language	English
Pages (from-to)	3-9
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	52
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4005(98)00248-2
Publication status	Published - 1998 Sep 15

Keywords

Avidin-biotin system
Biotin-labeled enzyme
Enzyme multilayer
Enzyme sensor
Glucose sensor
Lactate sensor

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction. / Anzai, Jun Ichi; Kobayashi, Yuka; Suzuki, Yasuhiro; Takeshita, Hiroki; Chen, Qiang; Osa, Tetsuo; Hoshi, Tomonori; Du, Xiao Yan.

In: Sensors and Actuators, B: Chemical, Vol. 52, No. 1-2, 15.09.1998, p. 3-9.

Research output: Contribution to journal › Article › peer-review

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title = "Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction",

abstract = "An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed.",

keywords = "Avidin-biotin system, Biotin-labeled enzyme, Enzyme multilayer, Enzyme sensor, Glucose sensor, Lactate sensor",

author = "Anzai, {Jun Ichi} and Yuka Kobayashi and Yasuhiro Suzuki and Hiroki Takeshita and Qiang Chen and Tetsuo Osa and Tomonori Hoshi and Du, {Xiao Yan}",

note = "Funding Information: This work was supported in part by Grants-in-Aid (Nos.09878206, 09558110 and 09237210: Electrochemistry of Ordered Interfaces) from the Ministry of Education, Science, Sports and Culture of Japan. ",

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AU - Anzai, Jun Ichi

AU - Kobayashi, Yuka

AU - Suzuki, Yasuhiro

AU - Takeshita, Hiroki

AU - Chen, Qiang

AU - Osa, Tetsuo

AU - Hoshi, Tomonori

AU - Du, Xiao Yan

N1 - Funding Information: This work was supported in part by Grants-in-Aid (Nos.09878206, 09558110 and 09237210: Electrochemistry of Ordered Interfaces) from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 1998/9/15

Y1 - 1998/9/15

N2 - An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed.

AB - An alternate and repeated deposition of avidin and biotin-labeled glucose oxidase (GOx) or lactate oxidase (LOx) on a solid surface gave protein multilayers through a strong binding between avidin and biotin. Spectrophotometric and gravimetric techniques using UV absorption and a quartz-crystal microbalance revealed that the protein multilayers are composed of avidin monolayers and enzyme monolayers which in turn are built in a layer-by-layer structure. The GOx and LOx enzymes were found to be catalytically active in the multilayer films, by means of a cyclic voltammetry. The response characteristics of the GOx- and LOx-modified sensors are discussed.

KW - Avidin-biotin system

KW - Biotin-labeled enzyme

KW - Enzyme multilayer

KW - Enzyme sensor

KW - Glucose sensor

KW - Lactate sensor

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Enzyme sensors prepared by layer-by-layer deposition of enzymes on a platinum electrode through avidin-biotin interaction

Abstract

Keywords

ASJC Scopus subject areas

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