Enzyme Sensors Based on an Ion-Sensitive Field Effect Transistor Coated with Langmuir-Blodgett Membranes: Use of Polyethyleneimine as a Spacer for Immobilizing a-Chymotrypsin

Jun Ichi Anzai, Shouryu Lee, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

An anti-neoplastic factor, dolabellanin A, inducing tumor lysis was purified from the albumen gland of a sea hare, Dolabella auricularia. Purified dolabellanin A was found to be a glycoprotein of 250 kilodaltons containing 4 subunits. This factor was half-maximally active to words a variety of tumor cells at 1—18 ng protein/ml and lysed tumor necrosis factor (TNF)-resistant tumor cells. Dolabellanin A was labile on heating, at low and high pH, and on treatment with urea, guanidine, sodium lauryl sulfate or trypsin, but not with 2-mercaptoethanol or periodate. Dolabellanin A completely inhibited the syntheses of deoxyribonucleic acid and ribonucleic acid by tumor cells within 1 h and caused their complete cytolysis within 18 h. Tumor lysis by dolabellanin A was not inhibited by anti-TNF antibody but was inhibited by certain sugars, suggesting that recognition of a sugar moiety is a key step in its induction of cytolysis. Dolabellanin A also prolonged the survival of mice bearing syngeneic MM46 ascitic tumors (p <0.001). These results suggest that dolabellanin A, found in an invertebrate the sea hare, is a new antitumor factor.

Original languageEnglish
Pages (from-to)3320-3322
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume37
Issue number12
DOIs
Publication statusPublished - 1989

Keywords

  • Langmuir-Blodgett membrane
  • a-chymotrypsin
  • enzyme sensor
  • ion-sensitive field effect transistor
  • polyethyleneimine
  • potentio-metric sensor

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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