Enzyme microcapsules with substrate selective permeability constructed via layer-by-layer polyelectrolyte self-assembly

Zi Xia Zhao, Xin Sheng Wang, Xia Qin, Qiang Chen, Jun ichi Anzai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Polyelectrolyte multilayer microcapsules with entrapped horseradish peroxidase (HRP) have been prepared via a layer-by-layer (LbL) self-assembly strategy of polycation and polyanion on CaCO3 microparticles as templates. Preparation conditions have been studied and optimized. Within the investigated ranges, use of buffer solutions with lower pH value or lower ionic strength in the buffer solution for polyelectrolyte self-assembly has resulted in thinner polyelectrolyte film and higher permeability of the microcapsules. For dissolving the CaCO3 templates, use of weakly acidic (pH 4.0) buffer solution in place of routinely used EDTA reagent has improved the catalytic activity of microcapsules. The HRP-containing microcapsules have exhibited catalytic activity to pyrogallol as substrate, while the catalytic activity to 2,2′-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) was severely suppressed. No less than 90% of the maximum enzymatic activity to pyrogallol has remained after 30 days. Promising prospects in various biocatalysis applications have been expected for the enzyme microcapsules, due to their selective permeability, stability and reusability.

Original languageEnglish
Pages (from-to)569-573
Number of pages5
JournalMaterials Science and Engineering C
Volume32
Issue number3
DOIs
Publication statusPublished - 2012 Apr 1

Keywords

  • Enzyme
  • Layer-by-layer self-assembly
  • Microcapsules
  • Permeability
  • Polyelectrolyte

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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