Enzyme-linked immunosorbent assay for IgG and IgM antibodies against human parvovirus B19: use of monoclonal antibodies and viral antigen propagated in vitro

Yaegashi Nobuo, Shiraishi Hiroyuki, Tada Kohtaro, Yajima Akira, Sugamura Kazuo

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Enzyme-linked immunosorbent assay (ELISA) systems for serum IgG and IgM antibodies to human parvovirus B19 were established by utilizing anti-B19 monoclonal antibodies (mAbs) and human plasma B19 antigen. The specificities of IgG and IgM ELISA were confirmed by indirect immunofluorescence staining and Western blot immunoassay with panel sera. The series of serum specimens obtained from two B19-infected patients were examined with ELISA. The IgM antibody titers increased quickly after the onset of the symptoms and returned to a negative range after five months. The IgG antibody titers also increased just after the increasing of IgM titers and the elevated levels continued for more than a year. We also established the same ELISA systems by utilizing in vitro propagated B19 antigen and similar results were obtained.

Original languageEnglish
Pages (from-to)171-181
Number of pages11
JournalJournal of Virological Methods
Volume26
Issue number2
DOIs
Publication statusPublished - 1989 Nov

Keywords

  • ELISA
  • Human parvovirus B19
  • Monoclonal antibody

ASJC Scopus subject areas

  • Virology

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