Enzyme-linked immunosorbent assay for bovine apolipoprotein A-IV

Yuji Takahashi, Hirotoshi Konishi, Kan Sato, Tsutai Oohashi, Toru Miyamoto

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The present report describes an enzyme-linked immunosorbent assay for bovine apolipoprotein (apo) A-IV. This assay was applied to the determination of its concentration and distribution in sera from cattle. The distribution of apoA-IV in lipoprotein fractions separated by ultracentrifugation was mostly recovered in the non-lipoprotein fractions (d>1.21 g/ml, 90%), but, in the case of gel filtration chromatography, apoA-IV was mainly eluted in HDL and non-lipoprotein fractions. The apoA-IV concentrations during early, mid- and late lactating stages in cows were significantly higher than during the nonlactating stage (p<0.05). From early to late lactating stages, the concentration of apoA-IV was unaltered. After 4 days of fasting, the concentration of plasma apoA-IV had decreased significantly (p<0.05) at days 3 and 4, and was returned to the basal level by 3 days of refeeding. These results suggested that the concentration of apoA-IV is modified by nutritional conditions.

Original languageEnglish
Pages (from-to)1199-1204
Number of pages6
JournalJournal of Veterinary Medical Science
Volume66
Issue number10
DOIs
Publication statusPublished - 2004 Oct

Keywords

  • Apolipoprotein A-IV
  • Bovine
  • ELISA
  • Lipoprotein

ASJC Scopus subject areas

  • veterinary(all)

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