Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine

Toshifumi Niwa, Kazuhiro Fujita, Toko Kasuya, Shigeo Ikegawa, Junichi Goto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A specific enzyme immunoassay (EIA) for ursodeoxycholic acid 7-N-acetylglucosaminides (UDCA-7-NAGs), novel metabolites of ursodeoxycholic acid, has been developed. The EIA was based upon antisera elicited against the glycine conjugated UDCA-7-NAG-bovine serum albumin conjugate and horseradish peroxidase-labeled-UDCA-7-NAG. The sensitivity was significantly improved by the utilization of the bridge heterologous combination system. An appropriate dose response curve of EIA for glycine conjugated UDCA-7-NAG was obtained in the range of 0.3-6ng/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related bile acids. The proposed method is useful for the determination of UDCA-7-NAGs in human urine with acceptable reliability.

Original languageEnglish
Pages (from-to)565-568
Number of pages4
Journalanalytical sciences
Volume12
Issue number4
DOIs
Publication statusPublished - 1996

Keywords

  • Bile acid
  • Enzyme immunoassay
  • Horseradish peroxidase
  • Ursodeoxycholic acid 7-N-acetylglucosaminide

ASJC Scopus subject areas

  • Analytical Chemistry

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